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Forum: Metagenomics 11-21-2016, 11:36 AM
Replies: 0
Views: 1,936
Posted By molbionerd
Concentrations of Post-indexed libraries

Hello all

I have been working with microbiome libraries using Illumina's recommended protocol and sequencing the V3-V4 regions. My question is about the post-indexed library concentrations. I am...
Forum: Ion Torrent 06-05-2016, 04:05 PM
Replies: 1
Views: 2,146
Posted By molbionerd
I think sonication is probably the way to go with...

I think sonication is probably the way to go with DNA as it is the least prone to bias. However, if you are set on using an enzymatic approach, you should be able to do it one of two ways. Either use...
Forum: Introductions 06-03-2016, 11:12 AM
Replies: 0
Views: 684
Posted By molbionerd

Just wanted to introduce myself, as that is what the site told me to do.

I am a user of Ion Torrent PGM and Illumina Miseq currently in the Genomics Facility. I have just started as the facility...
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