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adapter alignment annotation assembly atac-seq bam bbduk bbmap bbtools bioanalyzer bioconductor bioinfomatics bioinformactics bioinformatics blast bowtie bowtie2 bwa cancer chip-seq coverage cuffdiff cufflinks cuffmerge cummerbund deseq deseq2 differential expression edger error fastq fastqc fpkm gatk genome genomics hisat2 hiseq htseq-count illumina library preparation mapping metagenomics microbiome miseq nanopore nextera nextseq ngs normalization pacbio pipeline python quality rna rna-seq rnaseq rna seq sam samtools sequencing snp star tophat tophat2 trimmomatic truseq vcf velvet workshop

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