Forum: General
09-01-2019, 01:21 PM
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Replies: 1
Views: 2,470
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Forum: General
09-01-2019, 07:47 AM
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Replies: 1
Views: 2,470
Platform Comparison Table
Hello,
I am giving a general talk to a room of undergraduates soon. For it I will draw a 5-D (3-D plus color and size of points) graph comparing Illumina, Pac, Oxford and Ion. The purpose of the...
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Forum: Ion Torrent
08-30-2019, 11:52 AM
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Replies: 19
Views: 10,013
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Forum: General
04-05-2018, 10:15 AM
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Replies: 0
Views: 1,110
ABRF NGS Side Meeting 4/24
At ABRF 2018 Meeting in Myrtle Beach (https://conf.abrf.org/), there will be an ‘invitation only’ evening discussion on issues affecting Next Generation Sequencing cores. This survey...
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Forum: RNA Sequencing
03-09-2018, 05:27 AM
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Replies: 2
Views: 3,174
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Forum: RNA Sequencing
03-02-2018, 07:47 AM
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Replies: 2
Views: 3,174
RNA-seq in the NovaSeq
The smallest S1 kit is 100-cycles. With RNA-seq this results in a choice of single-end (100bp) or paired-end (2x50).
For the detection of splice junctions in, say, 300bp fragments...
Perhaps...
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Forum: Academic/Non-Profit Jobs
07-07-2016, 04:02 PM
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Replies: 0
Views: 931
St. Louis, Washington University
Job Type/Schedule
This opportunity is full-time and works approximately 40 hours per week. Hours are generally normal business hours but may vary due to the timing for experiments and other research...
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Forum: RNA Sequencing
04-12-2016, 03:45 AM
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Replies: 3
Views: 1,862
80% ethanol
Thanks to both of you?
These are shipped at room temp?
Is methylnick's concern about shipping a flammable a concern?
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Forum: RNA Sequencing
04-07-2016, 12:10 PM
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Replies: 3
Views: 1,862
Ship RNA @ RT in isoprop?
I have been asked a few times and have not been able to find a clear answer online. Is there any reason that RNA pellets cannot be shipped at room temp in 100% isopropanol?
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Forum: Academic/Non-Profit Jobs
08-26-2015, 06:55 AM
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Replies: 0
Views: 652
Meet me in St. Louis! (WashU: Tech II)
Job Type/Schedule
This position is full-time and works approximately 40 hours per week.
Department Name/Job Location
This position is for the Genome Technology Access Center for the Medical...
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Forum: Illumina/Solexa
03-18-2015, 05:11 AM
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Replies: 10
Views: 4,702
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Forum: Illumina/Solexa
03-17-2015, 01:16 PM
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Replies: 10
Views: 4,702
HiSeq 3000/4000 2x150 data
Hello wise ones,
Has anyone out there had any paired-end long read experience. We'd love to see the run data (Q30 graphs, etc).
S
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Forum: Bioinformatics
01-17-2014, 04:44 AM
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Replies: 4
Views: 1,316
I made this. Does it look right?
...
I made this. Does it look right?
http://i.imgur.com/mXjlwTU.png
It suggests that to get >95% 30-fold coverage I need an average coverage of 41...can that be right??
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Forum: Bioinformatics
01-16-2014, 09:36 AM
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Replies: 4
Views: 1,316
Advanced coverage predictor
Hi all,
Is there some kind of a Poisson calculator out there that will predict not only mean coverage but coverage at various percents? For example, given a human genome, what mean coverage do I...
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Forum: Sample Prep / Library Generation
01-30-2013, 06:17 AM
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Replies: 1
Views: 3,489
Pre-capture
We are looking at this pool as well, but have not yet made the decision to dive in (har-har). Has anyone had significant experience with pre-capture pooling?
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Forum: Academic/Non-Profit Jobs
11-08-2012, 09:47 AM
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Replies: 0
Views: 887
Computational Biologist, Washington Univ.
Job Description
The Genome Technology Access Center (https://gtac.wustl.edu/) at Washington University is looking for a computational biologist with several years of experience to work closely with...
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Forum: Core Facilities
10-07-2011, 08:01 AM
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Replies: 1
Views: 2,388
Need to Outsource
Hi all,
While we at GTAC have increased our capacity, we sometimes have more NGS than we can get done in a timely manner. Would you consider taking on some out-source work? If so could you...
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Forum: Illumina/Solexa
10-05-2011, 04:13 PM
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Replies: 5
Views: 3,025
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Forum: Events / Conferences
10-05-2011, 11:19 AM
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Replies: 0
Views: 1,921
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Forum: RNA Sequencing
09-15-2011, 03:11 AM
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Replies: 5
Views: 8,873
GTAC at WashU
Hello,
We provide, among other services, RNA-seq, with free analysis. Our prices can be seen here:
http://gtac.wustl.edu/pricing.php
Seth
__________________________________________
Seth...
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Forum: Illumina/Solexa
09-13-2011, 08:31 AM
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Replies: 0
Views: 1,742
Complete vs Illumina
Has there been any agnostic comparison done between the genome sequencing services of Complete Genomics vs. Illumina Genome Network (cost, data, turn-around time, etc)?
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Forum: 454 Pyrosequencing
09-11-2011, 07:58 AM
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Replies: 12
Views: 49,447
Forgive me...
I should have said 'soon' going to to 150 PE. Before the end of the year, I believe?
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Forum: Helicos / Direct Genomics
09-11-2011, 07:43 AM
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Replies: 19
Views: 42,488
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Forum: 454 Pyrosequencing
09-11-2011, 07:34 AM
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Replies: 2
Views: 2,590
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Forum: 454 Pyrosequencing
09-11-2011, 07:30 AM
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Replies: 12
Views: 49,447
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