Forum: Oxford Nanopore
02-27-2021, 07:49 AM
|
|
Replies: 11
Views: 6,798
|
|
Forum: Illumina/Solexa
09-23-2020, 10:47 AM
|
|
Replies: 26
Views: 12,478
|
|
Forum: Core Facilities
08-18-2020, 10:34 AM
|
|
Replies: 0
Views: 2,849
|
|
Forum: Service Providers
08-04-2020, 09:40 AM
|
|
Replies: 1
Views: 2,158
16S & 18S Deep Sequencing Request(s)
Hello,
We've done 16S/18S/ITS/amplicon sequencing for years, with good results - sometimes excellent (library dependent) - for both internal and external clients.
We're starting to see more...
|
|
Forum: Illumina/Solexa
04-24-2020, 12:48 PM
|
|
Replies: 26
Views: 12,478
|
|
Forum: Illumina/Solexa
04-23-2020, 09:46 AM
|
|
Replies: 9
Views: 2,328
|
|
Forum: Illumina/Solexa
04-23-2020, 09:32 AM
|
|
Replies: 1
Views: 1,744
|
|
Forum: Illumina/Solexa
04-18-2020, 08:31 AM
|
|
Replies: 1
Views: 1,744
MiSeq run failed; low clustering, but good QC
Have a strange situation, our latest MiSeq run failed for 16S - from a protocol (EMP @ ArgonneNatLabs) that we use all the time.
The clustering was unusually low (180 vs the expected 800) and we...
|
|
Forum: Sample Prep / Library Generation
04-01-2020, 03:09 PM
|
|
Replies: 12
Views: 3,946
We do a lot of environmental (soil, lake-water)...
We do a lot of environmental (soil, lake-water) and we do sometimes get off-target bands, but we don't bother cleaning-up, just pool, reduce volume using SpeedVac, KAPA and run them on the MiSeq. We...
|
|
Forum: Sample Prep / Library Generation
03-31-2020, 07:40 AM
|
|
Replies: 12
Views: 3,946
|
|
Forum: Sample Prep / Library Generation
03-31-2020, 06:36 AM
|
|
Replies: 3
Views: 5,195
Hi,
According to Eppendorf they have a...
Hi,
According to Eppendorf they have a protocol for this procedure: https://www.eppendorf.com/US-en/service-support/application-support/ngs-methods-for-epmotion/
Might contact them directly.
|
|
Forum: Sample Prep / Library Generation
03-31-2020, 06:11 AM
|
|
Replies: 0
Views: 1,264
epMotion Programming Forum?
Hi,
Is there a epMotion programming exchange forum anywhere? I'd really be interested in sharing programmes/methods and approaches with other users.
TIA
|
|
Forum: Sample Prep / Library Generation
03-31-2020, 06:06 AM
|
|
Replies: 12
Views: 3,946
|
|
Forum: Core Facilities
03-02-2020, 07:40 AM
|
|
Replies: 22
Views: 25,517
|
|
Forum: Core Facilities
03-02-2020, 07:25 AM
|
|
Replies: 22
Views: 25,517
|
|
Forum: Pacific Biosciences
12-04-2019, 07:56 PM
|
|
Replies: 6
Views: 6,477
|
|
Forum: Pacific Biosciences
12-04-2019, 01:07 PM
|
|
Replies: 2
Views: 4,657
|
|
Forum: Pacific Biosciences
11-19-2019, 05:45 AM
|
|
Replies: 6
Views: 6,477
|
|
Forum: Sample Prep / Library Generation
11-12-2019, 04:17 AM
|
|
Replies: 21
Views: 8,446
|
|
Forum: Sample Prep / Library Generation
11-11-2019, 07:26 PM
|
|
Replies: 21
Views: 8,446
|
|
Forum: Sample Prep / Library Generation
11-11-2019, 06:13 PM
|
|
Replies: 21
Views: 8,446
|
|
Forum: Sample Prep / Library Generation
10-30-2019, 08:20 AM
|
|
Replies: 2
Views: 1,820
(2x) Final Bead Clean-Up
Hello,
I have a general question regarding NGS library prep protocols; many of the protocols suggest using TWO rounds of bead clean-up at the end of the protocol. I am curious as to why TWO (2)...
|
|
Forum: Pacific Biosciences
10-06-2019, 07:15 AM
|
|
Replies: 17
Views: 12,833
|
|
Forum: Pacific Biosciences
10-06-2019, 07:09 AM
|
|
Replies: 6
Views: 6,477
|
|
Forum: Pacific Biosciences
10-03-2019, 01:54 PM
|
|
Replies: 6
Views: 6,477
SMRT Link 7.0 - HGAP
Has anyone gotten the HGAP assembler to work? It seems that it just barfs at different places constantly. Doesn't seem to be very robust at all.
I've used CANU before, so I'm going to try to go...
|
