Forum: Sample Prep / Library Generation
06-01-2020, 09:41 AM
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Replies: 5
Views: 3,705
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Forum: Illumina/Solexa
03-25-2020, 11:23 AM
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Replies: 5
Views: 2,178
This is probably a bit late for you, but I'll...
This is probably a bit late for you, but I'll chime in here with my own experience for those who come after. I needed more indexes than were available in any Illumina product. However, rather than...
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Forum: Illumina/Solexa
03-25-2020, 11:17 AM
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Replies: 2
Views: 2,468
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Forum: Illumina/Solexa
03-25-2020, 09:49 AM
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Replies: 1
Views: 1,465
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Forum: Bioinformatics
03-06-2018, 03:49 PM
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Replies: 2
Views: 1,128
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Forum: Bioinformatics
03-02-2018, 10:10 AM
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Replies: 2
Views: 1,128
find differences between two genomes
I have genomic sequencing data from two individuals. These are clones from the same cell line, but they have had a change made by homologous recombination. They should be identical, but one group of...
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Forum: Ion Torrent
04-11-2016, 11:03 AM
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Replies: 8
Views: 7,755
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Forum: Ion Torrent
03-01-2016, 11:22 AM
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Replies: 8
Views: 7,755
The Ion Torrent-branded kits are much more...
The Ion Torrent-branded kits are much more expensive and I haven't found them to be any better than others. I've used the NEB kit and it works fine. More recently I tried the Kapa kit and I like it...
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Forum: Ion Torrent
01-05-2016, 10:06 AM
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Replies: 122
Views: 112,998
You don't absolutely need the Chef. We have the...
You don't absolutely need the Chef. We have the PGM and the Proton, but no Chef, and we get along just fine. The Chef automates some things and saves time, but it's not necessary. I guess what I'm...
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Forum: Ion Torrent
09-30-2015, 02:55 PM
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Replies: 7
Views: 7,634
Ion Torrent is the company/brand name. Ion...
Ion Torrent is the company/brand name. Ion Torrent's sequencers are the PGM and the Proton. Functionally they are very similar in that they use the same chemistry and principles. The primary...
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Forum: Illumina/Solexa
09-25-2015, 02:10 PM
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Replies: 145
Views: 322,276
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Forum: Illumina/Solexa
09-22-2015, 04:18 PM
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Replies: 145
Views: 322,276
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Forum: 454 Pyrosequencing
01-12-2015, 11:14 AM
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Replies: 1
Views: 1,684
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Forum: 454 Pyrosequencing
01-12-2015, 11:09 AM
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Replies: 1
Views: 2,169
The problem with quantification using PicoGreen...
The problem with quantification using PicoGreen is that it will measure all the DNA is the sample, whether or not it can function in emPCR. Variation in ligation efficiency will therefore give you...
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Forum: Ion Torrent
04-10-2014, 11:12 AM
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Replies: 4
Views: 3,561
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Forum: The Pipeline
01-31-2014, 07:43 AM
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Replies: 7
Views: 11,677
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Forum: Bioinformatics
01-29-2014, 10:37 AM
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Replies: 1
Views: 1,751
MIRA failure
I recently finished the first run on our new Ion Torrent Proton. The sample is genomic DNA from a non-model organism so I wanted to start with a de novo assembly using the Assembler plugin for...
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Forum: RNA Sequencing
01-24-2014, 11:25 AM
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Replies: 24
Views: 16,187
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Forum: Ion Torrent
01-14-2014, 07:34 AM
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Replies: 2
Views: 4,997
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Forum: 454 Pyrosequencing
12-02-2013, 11:36 AM
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Replies: 4
Views: 2,974
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Forum: 454 Pyrosequencing
12-02-2013, 08:44 AM
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Replies: 4
Views: 2,974
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Forum: 454 Pyrosequencing
12-02-2013, 08:28 AM
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Replies: 6
Views: 2,900
Some questions to help us understand so we can...
Some questions to help us understand so we can help you:
Are you talking about the .fna and .qual files that are automatically created as part of the process, or are these files you created later...
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Forum: 454 Pyrosequencing
11-21-2013, 10:30 AM
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Replies: 6
Views: 3,115
It's all of the reads that passed the filters. ...
It's all of the reads that passed the filters. It contains all of the same reads as the .sff file; the difference is that the .fna file contains only the trimmed sequence information rather than all...
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Forum: 454 Pyrosequencing
11-08-2013, 08:23 AM
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Replies: 3
Views: 2,307
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Forum: 454 Pyrosequencing
10-31-2013, 09:04 AM
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Replies: 11
Views: 3,598
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