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Forum: RNA Sequencing 02-28-2018, 10:36 PM
Replies: 0
Views: 1,332
Posted By rajeev.vikram
Question Experimental Design - Single Cell RNA-Sequencing

Hi,

I am designing scRNA-Seq experiment on two cancer cell lines grown in specific conditions. I am curious as to what can be considered control in such experiments. In case of primary tumors, one...
Forum: Bioinformatics 02-07-2017, 07:46 PM
Replies: 6
Views: 6,836
Posted By rajeev.vikram
Hello Matt, According to my understanding,...

Hello Matt,

According to my understanding, the number of merged transcripts presented depends on the relative expression of the input transcript files. As the literature stares, " generate a...
Forum: Bioinformatics 06-03-2016, 08:30 AM
Replies: 0
Views: 1,303
Posted By rajeev.vikram
Post Basic and nieve Question in lncRNA analysis pipeline

Hi,

I have been analyzing RNA-Seq data sets of some Breast cancer cell lines to create a high confidence list of expressed lncRNAs. However as, I am new to NGS, I cannot figure out how do I filter...
Forum: RNA Sequencing 04-11-2016, 07:33 PM
Replies: 2
Views: 2,599
Posted By rajeev.vikram
Hi, Thanks for your reply, I have gone through...

Hi,
Thanks for your reply, I have gone through your lncRNApipe pipeline, however, when installing (I am trying to install in a custom directory, I am getting error with IO:Routine installation.
My...
Forum: RNA Sequencing 03-07-2016, 06:17 PM
Replies: 1
Views: 1,426
Posted By rajeev.vikram
How to download bulk fastq data for Analysis Pipeline

Hi,

I am looking for tutorial or step by step guide to download RNA-Seq data in bulk from DBs like GEO/SRA or Encode and directly put it in the analysis pipeline. (A kind of automated system which...
Forum: RNA Sequencing 01-04-2016, 09:45 PM
Replies: 2
Views: 2,599
Posted By rajeev.vikram
lncRNA study pipeline using STAR

Hi,

My Background:
I am new to NGS/RNA-seq and have been doing some background research. Now I suppose I have the basic theoretical knowledge and some specific questions, which I hope someone...
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