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Forum: Bioinformatics 05-13-2011, 10:42 AM
Replies: 1
Views: 1,716
Posted By kr067
Exclamation statistical significance for small fold changes

Hi everyone,

I have RNA seq data from the same tissue under 2 different experimental conditions and Im trying to find differentially regulated transcripts. I've used Cufflinks/ Cuffdiff to...
Forum: Sample Prep / Library Generation 08-05-2010, 02:42 PM
Replies: 2
Views: 2,079
Posted By kr067
Thanks for your reply. Both the ABI and the...

Thanks for your reply. Both the ABI and the Qiagen kits use sno RNAs as endogenous controls for the QRT PCR assays. These are much longer than 40 nt but my libraries are restricted to RNAs between 15...
Forum: Sample Prep / Library Generation 08-05-2010, 12:44 PM
Replies: 2
Views: 2,079
Posted By kr067
normalizing small RNA libraries

Hi all,
Im currently building small RNA libraries from the same animal tissue, but after different treatments. We're trying to see if small RNAs in this tissue are differentially regulated because...
Forum: Illumina/Solexa 05-06-2010, 07:22 AM
Replies: 145
Views: 291,852
Posted By kr067
Thank you sci_guy! that was really helpful!

Thank you sci_guy! that was really helpful!
Forum: Illumina/Solexa 05-05-2010, 12:56 PM
Replies: 145
Views: 291,852
Posted By kr067
paired end seq

Hi all,
Im new to paired end mRNA seq. Are the adapters for the paired end seq same as the genomic DNA adapters?
Thanks
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