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Forum: Illumina/Solexa 07-17-2011, 12:23 PM
Replies: 16
Views: 5,204
Posted By dmborek
Have you by any chance used Nextera kit for...

Have you by any chance used Nextera kit for preparing libraries?
Forum: Illumina/Solexa 04-07-2011, 01:58 PM
Replies: 4
Views: 2,555
Posted By dmborek
base composition vs. cluster generation

Could you elaborate how base composition affects cluster generation?

D.
Forum: Illumina/Solexa 04-07-2011, 01:54 PM
Replies: 2
Views: 1,993
Posted By dmborek
which instrument?

Was it GAII or HiSeq? What was clusters density?

D.
Forum: Illumina/Solexa 01-31-2011, 09:24 PM
Replies: 0
Views: 1,300
Posted By dmborek
results of mapping

I understand that percentage of reads mapped on reference genome depends both on quality of a library and on performance of a sequencing run.

I was wondering what percentage of mapped reads is...
Forum: Illumina/Solexa 09-27-2010, 03:48 PM
Replies: 18
Views: 7,077
Posted By dmborek
Are there any negatives of using longer inserts...

Are there any negatives of using longer inserts e.g. reduced number of clusters due to their larger size?
Anyone used even longer than 800 bp for PE (not mate pairs)?
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