Forum: Sample Prep / Library Generation
07-13-2016, 09:07 AM
|
|
Replies: 9
Views: 5,182
|
|
Forum: Sample Prep / Library Generation
09-18-2015, 06:03 AM
|
|
Replies: 4
Views: 3,608
|
|
Forum: Sample Prep / Library Generation
09-18-2015, 04:34 AM
|
|
Replies: 4
Views: 3,608
pooling libraries across multiple lanes
Dear SEQanswers experts - I have a set of 24 samples for RNAseq. The analyses I need to do is pairwise (i.e. 12 pairs of samples) for differential gene expression. I need to run them on a single...
|
|
Forum: Bioinformatics
05-22-2015, 04:42 PM
|
|
Replies: 15
Views: 5,310
|
|
Forum: Bioinformatics
05-22-2015, 04:27 PM
|
|
Replies: 15
Views: 5,310
hello - I'm stuck again. I downloaded and...
hello - I'm stuck again. I downloaded and untarred the iGenome file. trying with the above script - added in the paths, but the script trips up on the transcriptome_data/known and the bowtie2Index...
|
|
Forum: Bioinformatics
05-21-2015, 11:11 AM
|
|
Replies: 15
Views: 5,310
|
|
Forum: Bioinformatics
05-21-2015, 10:09 AM
|
|
Replies: 15
Views: 5,310
thanks - can you point me to a resource which...
thanks - can you point me to a resource which guides me through that part, i.e. how to incorporate these files into the tophat transcriptome index process? I'm a bit lost at the moment, and I have...
|
|
Forum: Bioinformatics
05-21-2015, 08:57 AM
|
|
Replies: 15
Views: 5,310
|
|
Forum: Bioinformatics
05-21-2015, 07:07 AM
|
|
Replies: 15
Views: 5,310
great - thanks for quick reply - i am not an...
great - thanks for quick reply - i am not an admin (and a fairly novice unix user, too). i've checked permissions on bowtie2 it says '-rwxrwx---' which i guess means user has no rights --- (last...
|
|
Forum: Bioinformatics
05-21-2015, 06:53 AM
|
|
Replies: 15
Views: 5,310
similar bowtie version issues
hi - i am having a similar problem with versions of bowtie/bowtie2 on a server. you mentioned:
"I have changed setting to make it work."
it would be great if you could expand on that...
...
|
|
Forum: RNA Sequencing
01-26-2015, 09:04 AM
|
|
Replies: 4
Views: 3,478
|
|
Forum: Bioinformatics
11-23-2012, 09:36 AM
|
|
Replies: 5
Views: 4,248
|
|
Forum: Bioinformatics
11-23-2012, 08:30 AM
|
|
Replies: 5
Views: 4,248
distinguish between gigabases and gigabytes: GB?
this is potentially a very dumb question: does everyone refer to gigabytes (of memory) and gigabases (of nucleotide sequence data) both as Gb in text? Or are there differences (GB and Gb)? Either way...
|
|
Forum: Ion Torrent
10-29-2012, 05:52 AM
|
|
Replies: 122
Views: 112,943
|
|
Forum: Ion Torrent
05-03-2012, 02:29 PM
|
|
Replies: 122
Views: 112,943
instrument reliability
sorry to stick my oar in but I'd like to ask the forum a (perhaps naive) question regarding reliability of illumina sequencers. speaking from anecdotal experience (i.e. chatting to various illumina...
|
|
Forum: Introductions
05-03-2012, 03:27 AM
|
|
Replies: 0
Views: 615
greetings
as suggested, i'm just saying hello. first day on seqanswers...now I just have to work out some good questions...
cheers,
Matt
KCL
|
