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Forum: Illumina/Solexa 05-24-2020, 08:24 AM
Replies: 8
Views: 6,272
Posted By mama
Q30 read2

How can you avoid getting a low Q30 of read2 (reverse read) in a 150bp paired end run when you sequence 10x sc 3'RNAseq?
Forum: Illumina/Solexa 11-05-2018, 10:13 PM
Replies: 8
Views: 3,339
Posted By mama
Which version of SAV enables to see the %...

Which version of SAV enables to see the % occupancy per tile. How can you check if the loading concentration looks good?
Many thanks!
Forum: Illumina/Solexa 01-21-2018, 07:32 AM
Replies: 23
Views: 8,840
Posted By mama
Misterc: could you give me the link of James...

Misterc: could you give me the link of James Hadfield article?
Forum: Vendor Forum 12-09-2015, 07:20 AM
Replies: 6
Views: 3,510
Posted By mama
We are currently using the Nextflex small RNA Seq...

We are currently using the Nextflex small RNA Seq kit v2 and we get huge amount of adapters dimers in our libraries. We use a PAGE TBE 10% gel after the PCR and most of the time the band at 150 bp is...
Forum: Sample Prep / Library Generation 08-18-2014, 10:41 AM
Replies: 2
Views: 4,761
Posted By mama
The Illumina SRA ladder is a DNA ladder and the...

The Illumina SRA ladder is a DNA ladder and the microRNA marker from NEB is a RNA ladder. That's the reason why they don't migrate the same way.
Forum: RNA Sequencing 03-12-2013, 11:52 AM
Replies: 4
Views: 1,803
Posted By mama
Thank you very much kwaraska for your answer. We...

Thank you very much kwaraska for your answer. We are also interested in preparing paired end Truseq stranded RNA seq with 200-300 bp inserts . In its protocol Illumina used dUTP for cDNA conversion...
Forum: RNA Sequencing 03-11-2013, 12:29 PM
Replies: 4
Views: 1,803
Posted By mama
Hi Kwaraska, Can you give me more...

Hi Kwaraska,

Can you give me more information about the protocol used by your customers?
Can you describe the steps until you get cDNA from your non-fragmented RNA?
Forum: Illumina/Solexa 10-01-2012, 11:39 AM
Replies: 11
Views: 3,107
Posted By mama
upper cut

Phillip, can you explain me in which step during the library prep do you do the "upper cut" ampures to remove the higher molecular weight product?
Did you try to do it in the chipSeq library prep?
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