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Forum: Illumina/Solexa 11-02-2015, 05:50 AM
Replies: 5
Views: 2,101
Posted By simsalabim
Hey fabp, so the sequence I wrote down is...

Hey fabp,

so the sequence I wrote down is the one that needs to be present AFTER all your PCR steps => the one that will be put on the sequencer. So originally you start with the reverse...
Forum: Illumina/Solexa 10-30-2015, 09:18 AM
Replies: 5
Views: 2,101
Posted By simsalabim
Hi, I'm a little confused why you reverse...

Hi,

I'm a little confused why you reverse complemented the sequence of the "nonread adapter".

just to make sure, here is the design as it should be put on the sequencer:
...
Forum: Illumina/Solexa 10-29-2015, 03:28 AM
Replies: 0
Views: 1,232
Posted By simsalabim
Question small RNA seq - sRNAbench

Hi,

I have two questions concerning the analysis of a small RNA seq data set.

1. The tool I would like to use to quantify miRNAs and possibly piRNAs is sRNAbench. This works well in "library...
Forum: Bioinformatics 03-13-2013, 04:48 AM
Replies: 6
Views: 2,409
Posted By simsalabim
Hi, I realize this thread is older but I get a...

Hi,
I realize this thread is older but I get a similar error message while using bfast for colorspace alignment.

My data consists of colorreads of length 75, dynamically trimmed down to >30 in...
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