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Forum: Sample Prep / Library Generation 04-01-2020, 04:26 AM
Replies: 3
Views: 837
Posted By Marc_Jones
Made it to CRAN yet?

Made it to CRAN yet?
Forum: Sample Prep / Library Generation 02-07-2020, 04:30 AM
Replies: 1
Views: 1,749
Posted By Marc_Jones
Did you end up trying?

Did you end up trying?
Forum: Bioinformatics 11-21-2019, 02:30 AM
Replies: 4
Views: 836
Posted By Marc_Jones
I would email all four authors...they do all have...

I would email all four authors...they do all have emails on the Article as sort of corresponding authors.
Forum: Oxford Nanopore 04-23-2019, 11:53 PM
Replies: 2
Views: 2,166
Posted By Marc_Jones
You could just add the index to the primers when...

You could just add the index to the primers when you order them.

or

SQK-RPB004 or SQK-PBK004 depending on your experiment.
Forum: RNA Sequencing 03-14-2019, 01:43 AM
Replies: 1
Views: 1,667
Posted By Marc_Jones
I would take 5mm x 5mm blocks of tissue and snap...

I would take 5mm x 5mm blocks of tissue and snap freeze them.
Macrodissect and homogenise in RLT using a hand homogeniser, or use a bead mill homogeniser.
Forum: Sample Prep / Library Generation 03-04-2019, 06:14 AM
Replies: 6
Views: 4,355
Posted By Marc_Jones
I've snap froze tissues without any loss of RNA...

I've snap froze tissues without any loss of RNA integrity. The biggest effect on RNA integrity is the time taken from sacrifice to freezing the tissue.

The main reason we froze was because wanted...
Forum: Oxford Nanopore 02-18-2019, 02:44 AM
Replies: 2
Views: 2,909
Posted By Marc_Jones
Which library prep method are you using? Have...

Which library prep method are you using? Have you read the protocol?
Forum: General 08-03-2018, 05:33 AM
Replies: 0
Views: 2,071
Posted By Marc_Jones
Biomek FXp

I have the opportunity to get a second hand BIOMEK FXp for library prep. What are peoples experience with them? Good, bad or indifferent? Shortfalls?

I haven't committed to anything and trying...
Forum: Illumina/Solexa 04-13-2018, 04:31 AM
Replies: 6
Views: 2,937
Posted By Marc_Jones
Thanks all

Thanks all
Forum: Illumina/Solexa 04-11-2018, 11:56 PM
Replies: 6
Views: 2,937
Posted By Marc_Jones
Edinburgher what multiplexing/pooling strategy do...

Edinburgher what multiplexing/pooling strategy do you use?

I would like to pool more than 24 samples...closer to 48 and use a NovaSeq but I don't think it's possible?
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