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Forum: Illumina/Solexa 05-15-2016, 06:42 PM
Replies: 0
Views: 1,151
Posted By Gaurav_genomics
Strands after fragmentation. Step 1.

At the step 1 of Illumina SBS, we fragment the DNA strandsand then add adapters followed by denaturing and ligation to the flow cell. Then based on polymerization reaction, how do we take care of the...
Forum: Metagenomics 08-06-2015, 01:28 AM
Replies: 0
Views: 1,259
Posted By Gaurav_genomics
Hello All.I have annotated my metagenomic dataset using MG-RAST and now want to gener

I have annotated the Metagenomic dataset using MG-RAST and now I want to generate the ".tree" phylogenetic tree file that can be further given as an input to QIIME for beta diversity calculation....
Forum: Bioinformatics 02-24-2014, 09:24 PM
Replies: 1
Views: 1,639
Posted By Gaurav_genomics
Kmer spectrum

http://s17.postimg.org/5zs1ekfl9/kmer.jpg

The kmer spectrum plots the number of distinct N-bp sequences as a function of coverage level, placing low-coverage (rare) sequences at left and...
Forum: Illumina/Solexa 11-05-2013, 08:39 PM
Replies: 1
Views: 1,328
Posted By Gaurav_genomics
TruSeq™ RNA-Seq

From where can I get a good literature to understand polyA based RNA capture in a more better manner.
Forum: Bioinformatics 03-12-2013, 08:30 PM
Replies: 2
Views: 2,973
Posted By Gaurav_genomics
Tophat [Errno 8] Exec format error

I have installed everything and all the necessary libraries required but its giving the error:

[Errno 8] Exec format error

The full output with the test data is as follows:

tophat -r 20...
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