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Forum: Bioinformatics 09-11-2017, 03:49 AM
Replies: 5
Views: 1,741
Posted By DeondeJager
Hi again Brian, I calculated the mismatch...

Hi again Brian,

I calculated the mismatch (or actually the match) rate from the .bam file using the mhist function in bbduk (mapping was done with bwa and of course the .bam files were produced...
Forum: Bioinformatics 08-28-2017, 10:56 AM
Replies: 5
Views: 1,741
Posted By DeondeJager
Thanks for the reply, Brian! I remember reading...

Thanks for the reply, Brian! I remember reading something about this fragmentation bias, now that you mention it, but I wasn't too sure if it applied to TruSeq as well. So I thought I'd rather be as...
Forum: Bioinformatics 08-28-2017, 12:16 AM
Replies: 5
Views: 1,741
Posted By DeondeJager
5' Kmers problem with whole genome re-sequencing data

Hi all,

I am having some issues with getting rid of kmers in my sequencing data sets. We have re-sequenced several African buffalo genomes on an Illumina HiSeq X machine, 150bp paired-end reads. I...
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