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Forum: Bioinformatics 07-20-2014, 10:35 AM
Replies: 3
Views: 2,328
Posted By Bitar
I have the same exact issue during round 5 ...

I have the same exact issue during round 5

Processing RECON family: 21802
- Saving elements to a file...
FastaDB::getSubstr - Error index out of bounds! (SeqID=gi|4517, offset=2237, length=327...
Forum: Sample Prep / Library Generation 11-06-2012, 12:43 PM
Replies: 1
Views: 1,627
Posted By Bitar
Sigma makes an RNAse free Ammonium Acetate, 7.5M...

Sigma makes an RNAse free Ammonium Acetate, 7.5M (A2706-100ml) which is what I use in my RNA preps. Works well.
As for your other question, not sure RiboZero would work on cDNA (double stranded, DNA...
Forum: RNA Sequencing 10-31-2012, 08:39 AM
Replies: 0
Views: 929
Posted By Bitar
creating a normalized cDNA library

Hi,
I am wondering what everyone uses to create normalized cDNA libraries (eukaryotic transcriptome). I've read about the Trimmer kit here from Evrogen, any others? Just looking for a quality kit...
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