Forum: Bioinformatics
05-06-2018, 03:01 PM
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Replies: 1
Views: 1,061
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Forum: Bioinformatics
03-11-2013, 01:46 PM
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Replies: 2
Views: 1,404
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Forum: Bioinformatics
03-11-2013, 09:03 AM
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Replies: 2
Views: 1,404
Genomer - software to simplify genome finishing
Hi Everyone,
I wrote some software to simplify the final steps in a genome project. Specifically finalizing the locations of contigs and annotations in an assembly. This software also makes it...
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Forum: De novo discovery
06-29-2010, 07:53 AM
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Replies: 2
Views: 3,873
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Forum: De novo discovery
06-28-2010, 12:36 PM
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Replies: 2
Views: 3,873
How to bridge genome gaps resulting from repeats?
We sequenced a Pseudomonas genome which resulted in several 454 scaffolds. From examining the sequence data it looks like there are many gaps in the sequence because of repeated rRNA genes. Newbler...
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Forum: Bioinformatics
06-11-2010, 01:50 PM
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Replies: 0
Views: 2,179
Segmentation fault in consed
I have assembled sequence data from half a 454 plate. The data was assembled with newbler and appears to have gaps due to problems resolving repeats e.g. repeated rRNA genes. I've heard that consed's...
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Forum: De novo discovery
01-23-2010, 04:04 PM
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Replies: 2
Views: 3,222
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Forum: 454 Pyrosequencing
01-23-2010, 04:03 PM
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Replies: 2
Views: 1,890
Rubber gasket vs. Molecular Markers
We're just about to do some 454 sequencing and de novo assembly of bacterial genomes. We're considering using molecular markers to sequence multiple different genomes instead of the rubber gasket to...
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Forum: De novo discovery
01-19-2010, 10:42 AM
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Replies: 2
Views: 3,222
Getting started with de novo genome sequencing
I'm new to genome sequencing and I'd like to ask for some general advice. We'd like to sequence as many P. fluorescens (Pf) strains as we can manage whilst try to get a reasonable assembly. We're...
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