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Forum: Sample Prep / Library Generation 04-04-2018, 02:40 AM
Replies: 5
Views: 3,539
Posted By sequencingfan
There is also a solution PCRClean Dx. Did anyone...

There is also a solution PCRClean Dx. Did anyone try this one?
Forum: Sample Prep / Library Generation 12-17-2014, 12:25 AM
Replies: 3
Views: 1,648
Posted By sequencingfan
What about applications in which we use only Pcr...

What about applications in which we use only Pcr to prepare libraries ( 16 s metagenomics,ion torrent ampliseq etc.).in my opinion most of reads for target region will start and end in the same...
Forum: Sample Prep / Library Generation 12-09-2014, 01:16 PM
Replies: 3
Views: 1,648
Posted By sequencingfan
PCR duplicates

Hello,

what is PCR duplicate in NGS sequencing? Is there a detailed explanation of this problem? What is the impact of PCR duplictes to data analysis.
Forum: Sanger/Dye Terminator 11-19-2014, 01:45 AM
Replies: 5
Views: 3,256
Posted By sequencingfan
Do You always set sequencing reactions on...

Do You always set sequencing reactions on chemistry version 1.1?
Did You check the protocol on instrument that is set up for version 1.1 not for 3.1?I think there are different mobility files etc....
Forum: Sample Prep / Library Generation 11-17-2014, 12:20 AM
Replies: 68
Views: 26,150
Posted By sequencingfan
I also have a question.what about non template...

I also have a question.what about non template adding nucleotides by reverse transcriptase.is there possibility that Rt add no 3 C but for example CCG.Is there possibility to use degenerate TSO?
Forum: Sample Prep / Library Generation 11-14-2014, 12:34 AM
Replies: 68
Views: 26,150
Posted By sequencingfan
In the article You linked in first post there is...

In the article You linked in first post there is Information that adaptor sequence is in the 3' end of dT oligo. Is that Right?
Forum: Sample Prep / Library Generation 11-12-2014, 03:32 AM
Replies: 68
Views: 26,150
Posted By sequencingfan
Thanks for information. Is there a special...

Thanks for information. Is there a special protocol for preparing libraries from cffDNA and is this protocol include shearing DNA after extraction or cffDNA is ready to use as it is (200 bp fragments...
Forum: Sample Prep / Library Generation 11-11-2014, 06:48 AM
Replies: 68
Views: 26,150
Posted By sequencingfan
Hi, is Your product suitable for cff DNA...

Hi,

is Your product suitable for cff DNA form maternal blood?
Forum: Sanger/Dye Terminator 11-11-2014, 06:27 AM
Replies: 5
Views: 3,256
Posted By sequencingfan
Hello, in my opinion the average signal...

Hello,

in my opinion the average signal strenght for all bases is very low ( below 100 ) and the software pull up the background to maximaze the real signal thats why You get double signal but...
Forum: Sanger/Dye Terminator 11-06-2014, 11:58 PM
Replies: 1
Views: 2,530
Posted By sequencingfan
low signal for one base

Sometimes i have problem with signal intensity for one base.The average signal for all bases is good. It is similar problem to G after A but it not always G that have very low signal.
Any...
Forum: Sample Prep / Library Generation 11-06-2014, 02:59 PM
Replies: 4
Views: 2,073
Posted By sequencingfan
I read in some article that loop adapters are...

I read in some article that loop adapters are more effective during ligation and we can expect more mapped reads to reference genome.
Forum: Sample Prep / Library Generation 11-06-2014, 03:04 AM
Replies: 4
Views: 2,073
Posted By sequencingfan
NebNext Adaptors for illumina

Hi all,

anyone know wchich adapters are better for whole genome sequencing, I'm thinking about NebNext loop adapters. Are they better than standard Y shape adapters.?
Forum: Vendor Forum 11-05-2014, 12:32 AM
Replies: 3
Views: 7,311
Posted By sequencingfan
I have question. Is nextflex compatible with Ion...

I have question. Is nextflex compatible with Ion proton sequencer?Do You provide adapters and other components separetly?
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