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Search: Posts Made By: smoics
Forum: Bioinformatics 11-20-2019, 12:09 PM
Replies: 5
Views: 952
Posted By smoics
I don't know our computer's IP address. Found...

I don't know our computer's IP address. Found directions for accessing it via the BIOs settings; however, I don't seem to have the IPMI configuration option & didn't manage to find it elsewhere. So...
Forum: Bioinformatics 11-19-2019, 02:16 PM
Replies: 5
Views: 952
Posted By smoics
Is that something I would need to have already...

Is that something I would need to have already set up? I've never done that before.
Forum: Bioinformatics 11-19-2019, 11:11 AM
Replies: 5
Views: 952
Posted By smoics
Nvidia drivers login loop plus flickering/blinking screen-Ubuntu 18.04 Linux

Got stuck in the login loop. I hard restarted the computer....went into the terminal and was having some path issues (I wanted to uninstall nvidia drivers). Ended up rebooting thru the terminal--it...
Forum: Bioinformatics 07-10-2019, 01:39 PM
Replies: 1
Views: 990
Posted By smoics
Fixed this by clearing some memory. Thanks for...

Fixed this by clearing some memory. Thanks for reading. Now onto tackling the rest of the install.
Forum: Bioinformatics 07-09-2019, 01:37 PM
Replies: 1
Views: 990
Posted By smoics
docker installation for Guppy basecaller

Linux
Ubuntu 18.04
nvidia-docker2 (2.0.3+docker18.09.7-3).
Nvidia driver 410.57
cuda 10.1

error occurs after: $ sudo docker run --runtime=nvidia --rm nvidia/cuda nvidia-smi

docker: Error...
Forum: Bioinformatics 12-11-2018, 06:04 AM
Replies: 1
Views: 624
Posted By smoics
post de novo assembly reference mapping?

I am curious if there is a program/app that would do post de novo assembly reference mapping--essentially breaking down the full scaffold into chunks and reference mapping it post-assembly.

I do...
Forum: Sample Prep / Library Generation 11-06-2018, 10:39 AM
Replies: 2
Views: 1,022
Posted By smoics
DNA cleanup kits

Hi. Looking to improve 260/230 ratios. I was told bead based DNA cleanup methods work better than column based. Other than AMPure beads, do you have any recommendations for this? Thanks.
Forum: Sample Prep / Library Generation 11-01-2018, 01:37 PM
Replies: 3
Views: 967
Posted By smoics
qubit standards left at RT overnight

Are the standards okay after having been left out overnight at RT?

Forgot to put them away--Just curious if they are still usable or if I should order a replacement.
Forum: Sample Prep / Library Generation 10-31-2018, 01:39 PM
Replies: 2
Views: 2,433
Posted By smoics
Agilent tech support is really helpful with...

Agilent tech support is really helpful with fixing these problems, if you are unsure how to do so. (Via phone or email them the run file)
Forum: Illumina/Solexa 10-10-2018, 06:26 AM
Replies: 0
Views: 690
Posted By smoics
low ending sample concentrations

My sample concentrations post-library prep are a lot lower using the Flex DNA kit compared to what I've had in the past using the XT kit. Is this likely loss during bead clean up (as this kit has...
Forum: Illumina/Solexa 09-27-2018, 07:03 AM
Replies: 1
Views: 803
Posted By smoics
BluePippin use w/ Nextera Kits

Always used BluePippin with XT kit, as a two-step size selection. Trying out the new Flex kit. Since the protocol calls for a set/given amount of beads in the Flex kit, will the BluePippin size...
Forum: RNA Sequencing 01-14-2016, 02:05 PM
Replies: 2
Views: 2,861
Posted By smoics
This is normal. Many people modify the protocol...

This is normal. Many people modify the protocol to increase the number of PCR cycles like this.
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