Forum: Illumina/Solexa
03-23-2011, 08:52 AM
|
|
Replies: 32
Views: 24,173
|
|
Forum: Illumina/Solexa
03-23-2011, 02:08 AM
|
|
Replies: 32
Views: 24,173
I don't think you can use the standard Ampure...
I don't think you can use the standard Ampure cleaning protocol, it removed most of the <75 bp fragments when I used it. 70bp DNA in combination with ~75bp adapters might work however, see attachment.
|
|
Forum: Illumina/Solexa
08-19-2010, 08:57 AM
|
|
Replies: 43
Views: 135,976
|
|
Forum: Illumina/Solexa
08-19-2010, 02:48 AM
|
|
Replies: 43
Views: 135,976
|
|
Forum: Illumina/Solexa
07-27-2010, 05:23 AM
|
|
Replies: 19
Views: 10,270
|
|
Forum: Sample Prep / Library Generation
07-27-2010, 01:12 AM
|
|
Replies: 10
Views: 5,167
Did you tile your bait design?
In another...
Did you tile your bait design?
In another thread I posted a question on the bait to sample ratio (http://seqanswers.com/forums/showthread.php?t=6091)
From your answer I get that this ratio is...
|
|
Forum: Sample Prep / Library Generation
07-26-2010, 06:15 AM
|
|
Replies: 5
Views: 7,210
I already have about 500ng of template, so i...
I already have about 500ng of template, so i think I don't really need to do the pre-hyb PCR. For qPCR the pre-hyb PCR is however required, since these primers only anneal to the extended PCR...
|
|
Forum: Sample Prep / Library Generation
07-23-2010, 06:20 AM
|
|
Replies: 1
Views: 3,925
|
|
Forum: Sample Prep / Library Generation
05-14-2010, 07:55 AM
|
|
Replies: 5
Views: 7,210
Could you further reduce the pre-hyb PCR cycle...
Could you further reduce the pre-hyb PCR cycle number, e.g to 3 rounds of PCR using PE primers?
I suppose theoretically 3 rounds is the minimum, since you need the symmetric PCR product mentioned...
|
