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Forum: Bioinformatics 05-26-2017, 06:59 AM
Replies: 0
Views: 789
Posted By Aragion
Alignment and assembly of metatranscriptomic paired-end reads


I have the sets of metatranscriptomic Illumina paired-end reads. I need to align them against the database of plant virus genomes (about 55000 sequences) and calculate coverage for each...
Forum: Bioinformatics 02-03-2017, 04:02 AM
Replies: 1
Views: 1,990
Posted By Aragion
Assembly of metatranscriptomic data

I have a set of reads produced by metatranscriptomic cDNA sequensing of the total RNA sample. I need to assemble the RNA-virus genome, which was detected in this sample by the...
Forum: Bioinformatics 12-20-2016, 09:08 AM
Replies: 2
Views: 1,795
Posted By Aragion
Low tophat2 mapping of paired-end reads

Hello. I'm trying to map paired-end reads on the reference scaffold using tophat2, but the percentage of mapped reads is almost 0%.

Left reads: Input: 9526882
Mapped: 224 ( 0.0% of input)
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