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Forum: Literature Watch 06-21-2012, 12:34 PM
Replies: 19
Views: 14,280
Posted By linampli
hi, to get back to the library prep, I am now...

hi,
to get back to the library prep, I am now finalising a procedure utilising LinDA for Illumina library prep. With the modified protocol one should get an Illumina library directly with LinDA,...
Forum: Literature Watch 06-21-2012, 12:28 PM
Replies: 19
Views: 14,280
Posted By linampli
hi, Did you purify the DNA after LinDA with...

hi,
Did you purify the DNA after LinDA with Qiagen columns or SPRI beads? I found that the T7 poly A primer doesnt get completely removed with column purification, this leads to interference in...
Forum: Literature Watch 04-12-2012, 06:33 AM
Replies: 19
Views: 14,280
Posted By linampli
someone pointed out a mistake in the...

someone pointed out a mistake in the amplification protocol, in the reagent list it is given as 1mM of rATP, rGTP, rCTP, rUTP. It should be 100 mM each as is supplied by the kit, please do not adjust...
Forum: Literature Watch 04-09-2012, 05:22 AM
Replies: 19
Views: 14,280
Posted By linampli
the detailed protocol is in nature protocols...

the detailed protocol is in nature protocols where the exact reaction volumes are given.
1. Unfortunately Promega seems to have discontinued SAP, however, the thermostable phosphatase seems to be a...
Forum: Literature Watch 03-28-2012, 06:36 AM
Replies: 19
Views: 14,280
Posted By linampli
linear amplification

the detailed protocol is in Nature protocols
Nat Protoc. 2012 Jan 26;7(2):328-38. doi: 10.1038/nprot.2011.447.
Single-tube linear DNA amplification for genome-wide studies using a few thousand...
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