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Forum: Literature Watch 06-15-2012, 10:22 PM
Replies: 19
Views: 14,280
Posted By a3911145
LinDA

Hi, I have seem the same phenomenon in the qPCR. But whether it's a bias or not, is under question. If the amount of gene B is higher in the starting material, it may amplify more after LinDA than A....
Forum: Literature Watch 06-05-2012, 07:24 AM
Replies: 19
Views: 14,280
Posted By a3911145
I am a PHD student from China Similar to...

I am a PHD student from China
Similar to thedavid, I think I have the same problem in LinDA: very low efficiency of the‘T’ tailing or low efficiency of attaching T7 primer.
I choose another...
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