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Forum: Sample Prep / Library Generation 01-13-2019, 11:59 AM
Replies: 15
Views: 20,379
Posted By aengstrom
Thanks for the answer nucacidhunter! I have...

Thanks for the answer nucacidhunter! I have checked now once again and the reply from Twist is: "I confirm again that both of our End-repair and A-tailing reactions are happening at 65C. Since our...
Forum: Sample Prep / Library Generation 01-12-2019, 12:08 PM
Replies: 0
Views: 843
Posted By aengstrom
8 nt Universal Blockers (blocking oligos) for 10 bp index?

We have used 8 bp xGen Universal Blockers - TS Mix from IDT for 8 bp indexed adapters from IDT. We are now switching to 10 bp indexed adapters but have a large amount of 8 bp blockers left...
Forum: Sample Prep / Library Generation 12-21-2018, 10:33 AM
Replies: 15
Views: 20,379
Posted By aengstrom
Thanks for the answer, appreciated! The reason...

Thanks for the answer, appreciated! The reason for me asking is that I am currently using the library prep (and target capture) from Twist Bioscience, exome and custom panel protocol. The current...
Forum: Sample Prep / Library Generation 12-18-2018, 10:43 PM
Replies: 15
Views: 20,379
Posted By aengstrom
End-repair at 65 degrees?

Does anyone know of an enzyme that end-repairs at 65 degrees?
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