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Forum: Illumina/Solexa 03-27-2017, 06:01 AM
Replies: 4
Views: 1,052
Posted By thermophile
you could order the primers that hit phix and put...

you could order the primers that hit phix and put those with your custom primers in the custom primer wells.

These are the primers that should hit phiX (I haven't tested them but this is what FAS...
Forum: Illumina/Solexa 03-24-2017, 09:43 AM
Replies: 4
Views: 1,052
Posted By thermophile
Have you tried new aliquots of the primers? PCR...

Have you tried new aliquots of the primers? PCR would work but sequencing fail if the primers are mixed up (R1 in R2 well, R2 in R1 well)
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