Forum: Metagenomics
08-24-2017, 04:36 AM
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Replies: 0
Views: 4,513
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Forum: Illumina/Solexa
02-15-2017, 01:04 AM
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Replies: 5
Views: 1,474
Thanks for your answer.
From my experience...
Thanks for your answer.
From my experience with the HiSeq, the raw sequences i got included barcodes and pcr primers (which makes sense, since they have been sequenced after all).
This indicates...
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Forum: Illumina/Solexa
02-14-2017, 02:21 PM
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Replies: 5
Views: 1,474
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Forum: Sample Prep / Library Generation
02-14-2017, 06:46 AM
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Replies: 0
Views: 972
batch tool for checking primers
Hi, i am about to order 400 barcoded primers (100 barcodes + 2 directions x 2 Genes of Interest).
Usually i check my primers with the OligoAnalyzer by IDT, but they dont accept the upload of fasta...
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Forum: Illumina/Solexa
01-15-2015, 03:40 AM
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Replies: 2
Views: 1,827
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Forum: Illumina/Solexa
01-15-2015, 03:28 AM
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Replies: 0
Views: 1,006
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Forum: Illumina/Solexa
12-18-2014, 09:04 AM
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Replies: 3
Views: 1,710
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Forum: Illumina/Solexa
12-18-2014, 04:36 AM
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Replies: 3
Views: 1,710
Removal of adapters and barcodes
Hi!
I want to compare an existing result from QIIME to cd-hit-otu-illumina.
cd-hit asks
"Barcodes should be removed. All the tags must start at 5' with the same rRNA primer shared by all...
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Forum: Bioinformatics
01-28-2014, 07:34 AM
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Replies: 366
Views: 202,026
Thanks for the quick answer.
My 460 million...
Thanks for the quick answer.
My 460 million reads were processed over night without troubles. I was just impatient.
I have paired end data, and it seems like some of the samples have...
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Forum: Bioinformatics
01-27-2014, 08:06 AM
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Replies: 366
Views: 202,026
Hi there!
Three quick questions....
1....
Hi there!
Three quick questions....
1. What is the maximum amount of data fastqc can handle?
I am trying to analyze a huge concatenated sample of illumina data, but its stuck @"Starting...
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Forum: Bioinformatics
09-16-2013, 05:44 AM
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Replies: 2
Views: 8,801
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