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Forum: Bioinformatics 03-25-2014, 04:58 AM
Replies: 0
Views: 1,651
Posted By Joshua_HIT
RefFreeEWAS help

Hi, these days I need to process some 450k methylation beta value with a R package RefFreeEWAS. But I can not understand the example given by the package instruction.

in the example, finally I...
Forum: Bioinformatics 12-14-2013, 10:10 PM
Replies: 2
Views: 2,041
Posted By Joshua_HIT
Thank you so much ! I was a student of computer...

Thank you so much ! I was a student of computer science, a totally freshman in Biology. it seems that I have been searching in wrong direction.

Thanks for your advise.
Forum: Bioinformatics 12-14-2013, 03:39 AM
Replies: 2
Views: 2,041
Posted By Joshua_HIT
How to use ClustalO?

Nice to meet you all!

I want to extract a consensus transcriptome for 4 species. Now I have de novo assemblied all transcripts for 4 species. I want to use Clust-Omega to finish Multiple Sequences...
Forum: Bioinformatics 12-08-2013, 01:36 AM
Replies: 4
Views: 2,357
Posted By Joshua_HIT
Thanks for your advise. I would have a look on...

Thanks for your advise. I would have a look on that!
Forum: Bioinformatics 12-08-2013, 01:35 AM
Replies: 4
Views: 2,357
Posted By Joshua_HIT
you are totally right. I am a new freshman on...

you are totally right. I am a new freshman on biology, I indeed need more patient! Thanks!!
Forum: Bioinformatics 12-07-2013, 05:06 AM
Replies: 4
Views: 2,357
Posted By Joshua_HIT
Tophat2 stucked

Hi:

I want to run tophat2 on four sets of data(human chimp gibbon gorilla). For human I downloaded hg19 from USCS in which Bowtie2 index has been included, so human has been finished successfully....
Forum: De novo discovery 12-07-2013, 04:54 AM
Replies: 3
Views: 3,874
Posted By Joshua_HIT
Hi I have tried to use GMAP as the downstream...

Hi
I have tried to use GMAP as the downstream software for Trinity's output. After that I got sam documents. My classmates said the sam documents can be treated by any MSA software, but I don't know...
Forum: Bioinformatics 10-18-2013, 11:13 PM
Replies: 2
Views: 1,341
Posted By Joshua_HIT
I think it means operations applied to finish the...

I think it means operations applied to finish the Mapping procedue, "M" means Match, "D"means Delete,"I" means insert, and so on.

I am using GMAP these days, but I did not reviece a result like...
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