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Forum: 454 Pyrosequencing 06-14-2010, 02:19 AM
Replies: 14
Views: 10,481
Posted By Aero
Hi AKroxy, Sorry for the typo and thank you...

Hi AKroxy,

Sorry for the typo and thank you for pointing it out...I actually meant a T-overhang for the adaptor. And it's a 3' A-overhang for the insert.
Forum: 454 Pyrosequencing 06-13-2010, 10:55 PM
Replies: 14
Views: 10,481
Posted By Aero
Rapid Library Adpators

I thought there should be a 3' A-overhang after the key sequence for TA-ligation of insert and adaptor...is that correct?
Forum: Illumina/Solexa 10-19-2009, 01:39 AM
Replies: 11
Views: 10,042
Posted By Aero
Hi, Our lab has similarly made our own...

Hi,

Our lab has similarly made our own adaptors with the phosphorothioate linkage (Sanger Nature Methods paper) and they work fine on the GAIIx.
Forum: Illumina/Solexa 06-23-2009, 01:03 AM
Replies: 6
Views: 5,068
Posted By Aero
Ultrapure ligase from Enzymatics

Hi,

I am curious if anyone has tried the "ultrapure ligase" (as in the Quail et al, Nature Methods paper) from Enzymatics. Thanks!

A large genome center's improvements to the Illumina...
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