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Forum: Illumina/Solexa 04-12-2017, 06:07 AM
Replies: 19
Views: 3,210
Posted By ECO
Anyone doing significant multiplexing should be...

Anyone doing significant multiplexing should be using dual indicies, which reduces this problem dramatically (making swapped reads go to Undetermined while demultiplexing).

Interested to see if...
Forum: Sample Prep / Library Generation 02-01-2017, 10:41 AM
Replies: 5
Views: 774
Posted By ECO
Anyone tried this? ^^ Seems gloriously too...

Anyone tried this? ^^

Seems gloriously too good to be true.
Forum: Sample Prep / Library Generation 01-31-2017, 07:22 PM
Replies: 5
Views: 774
Posted By ECO
Without a 2' -OH, the DNA won't shear. ...

Without a 2' -OH, the DNA won't shear.

Magnesium is in high concentrations in every PCR buffer also.
Forum: Academic/Non-Profit Jobs 12-06-2016, 07:42 AM
Replies: 1
Views: 539
Posted By ECO
Moving to Academic Jobs

Moving to Academic Jobs
Forum: Vendor Forum 12-06-2016, 07:41 AM
Replies: 1
Views: 1,129
Posted By ECO
Moving to Vendor forum

Moving to Vendor forum
Forum: Pacific Biosciences 11-07-2016, 03:44 PM
Replies: 2
Views: 1,980
Posted By ECO
I'd be interested too. I'm pretty sure the chip...

I'd be interested too. I'm pretty sure the chip is now the machine... :)
Forum: Pacific Biosciences 10-14-2016, 10:10 AM
Replies: 9
Views: 1,039
Posted By ECO
@Genomax...the logical explanation is that the...

@Genomax...the logical explanation is that the SMRTcell production process is dramatically more complicated than all other sequencing consumables, ZMW size and uniformity, the micromirror shape,...
Forum: Pacific Biosciences 10-13-2016, 11:59 AM
Replies: 9
Views: 1,039
Posted By ECO
Are you referring to just successful loading? Or...

Are you referring to just successful loading? Or some other metrics (RL, accuracy)?
Forum: Bioinformatics 09-27-2016, 01:55 PM
Replies: 15
Views: 1,808
Posted By ECO
Agreed. Closed.

Agreed. Closed.
Forum: 454 Pyrosequencing 09-08-2016, 08:26 AM
Replies: 5
Views: 4,446
Posted By ECO
This is a bummer...just tweeted a link to this...

This is a bummer...just tweeted a link to this thread to try to help.
Forum: Vendor Forum 08-23-2016, 01:49 PM
Replies: 11
Views: 2,496
Posted By ECO
Moving to Vendor Forum where this type of post...

Moving to Vendor Forum where this type of post belongs...
Forum: Pacific Biosciences 08-03-2016, 11:12 AM
Replies: 50
Views: 8,721
Posted By ECO
I don't have a ton of time to write all the...

I don't have a ton of time to write all the things I want to. However, I know a couple things.

1. PacBio folk are the smartest in the industry, period.

2. They learned their lesson with...
Forum: Pacific Biosciences 08-03-2016, 09:00 AM
Replies: 50
Views: 8,721
Posted By ECO
No one blocked you here, or slowed you down. ...

No one blocked you here, or slowed you down. Your paranoia is quite amusing. :D

The simplest explanation (https://en.wikipedia.org/wiki/Occam's_razor) is that, since you're at a University, that...
Forum: Sample Prep / Library Generation 07-28-2016, 02:48 PM
Replies: 7
Views: 6,259
Posted By ECO
IME, Klenow Exo- is unnecessary and only was ever...

IME, Klenow Exo- is unnecessary and only was ever included because the very very first library kits from Solexa had it in there. T4 pol is a monster at end removal and fill in. The blunting phase...
Forum: Vendor Forum 06-17-2016, 12:10 PM
Replies: 8
Views: 3,863
Posted By ECO
I found "75x on-target coverage" but that doesn't...

I found "75x on-target coverage" but that doesn't speak to uniformity or gaps etc....
Forum: Vendor Forum 06-17-2016, 12:08 PM
Replies: 8
Views: 3,863
Posted By ECO
I would love to know the technical details of the...

I would love to know the technical details of the assay:


--sequencing config (PE, SE, 75,100,150,???)
--Enrichment technology used
--Regions targeted
--Depth of sequencing, ie how many...
Forum: Oxford Nanopore 05-16-2016, 10:10 AM
Replies: 7
Views: 2,442
Posted By ECO
Can you do some simulations with the error rates...

Can you do some simulations with the error rates and length distributions of the system?

Having spent some time in the single molecule sampleprep world, the challenge with obtaining "pure" plant...
Forum: Bioinformatics 05-16-2016, 10:07 AM
Replies: 5
Views: 719
Posted By ECO
Learning both is great. Python+Pandas is pretty...

Learning both is great. Python+Pandas is pretty powerful, but (IME) is harder to learn than R as the syntax of pands, and the intelligent default behaviors are not as robust as they are in R.

I...
Forum: General 05-02-2016, 01:02 PM
Replies: 2
Views: 589
Posted By ECO
More info and a link would help.

More info and a link would help.
Forum: Vendor Forum 04-28-2016, 12:57 PM
Replies: 2
Views: 1,716
Posted By ECO
Moving to the Vendor Forum, where commercial...

Moving to the Vendor Forum, where commercial posts belong.
Forum: Sample Prep / Library Generation 03-08-2016, 12:23 PM
Replies: 2
Views: 876
Posted By ECO
Oh and you crossposted it a few times...tsk tsk :(

Oh and you crossposted it a few times...tsk tsk :(
Forum: Sample Prep / Library Generation 03-08-2016, 12:23 PM
Replies: 2
Views: 876
Posted By ECO
Sounds like someone's Lander-Waterman homework?

Sounds like someone's Lander-Waterman homework?
Forum: Vendor Forum 03-04-2016, 12:22 PM
Replies: 7
Views: 1,562
Posted By ECO
Agreed with blancha. Changing the title, but...

Agreed with blancha. Changing the title, but leaving it.
Forum: Vendor Forum 02-10-2016, 10:05 AM
Replies: 1
Views: 910
Posted By ECO
Wow cool! You wouldn't be their sales manager...

Wow cool! You wouldn't be their sales manager (http://bit.ly/20LJTrH) would you? :rolleyes:

Multiple other users pointed this out...so you're not going to fool anyone. Moving this to the Vendor...
Forum: Introductions 02-04-2016, 11:54 AM
Replies: 6
Views: 997
Posted By ECO
Hey guys sorry I missed this. Just email me...

Hey guys sorry I missed this. Just email me admin at seqanswers.com and we can continue the conversation there.

The PM thing prevents you from using the PM system until you've made a post that...
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