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Forum: Polonator 10-14-2010, 02:17 PM
Replies: 1
Views: 7,127
Posted By Kevin McCarthy
Rubenken: Thanks for posting. The Polonator is...

Rubenken: Thanks for posting. The Polonator is still commercially available, but despite its low upfront and recurring cost, its throughput has lagged behind that of the two major players. That...
Forum: Polonator 05-06-2010, 01:51 AM
Replies: 9
Views: 17,858
Posted By Kevin McCarthy
Yes, we have over a dozen Polonators in the...

Yes, we have over a dozen Polonators in the field. In some cases users are still learning how to use them (library prep alone is a bit complex); in other cases, they are in successful production. ...
Forum: Polonator 02-08-2010, 04:42 AM
Replies: 9
Views: 17,858
Posted By Kevin McCarthy
seqgirl77: We have several approaches to...

seqgirl77: We have several approaches to performing cyclical ligation, but none are yet robust enough to release. SBS (polymerase) is another route, but again, not yet ready for release. One item...
Forum: Polonator 06-09-2009, 04:01 AM
Replies: 42
Views: 31,446
Posted By Kevin McCarthy
New Thread opened

All: A new thread (http://seqanswers.com/forums/showthread.php?t=1670; "Updated Polonator ready for launch!") has been opened, since we are now accepting orders for the Polonator. My apologies to...
Forum: Polonator 06-09-2009, 03:47 AM
Replies: 9
Views: 17,858
Posted By Kevin McCarthy
To answer genseq's question, we are at the same...

To answer genseq's question, we are at the same point as when we updated the Polonator web site with the FAQ a few weeks ago. Two groups are working on cyclical ligation approaches, and the progress...
Forum: Polonator 08-25-2008, 06:54 AM
Replies: 42
Views: 31,446
Posted By Kevin McCarthy
I've attached a PDF detailing the Polonator...

I've attached a PDF detailing the Polonator initial biochemistry from a graphical perspective. This is a paired end tag approach, and is of necessity more complex than sequencing single tags, or...
Forum: Polonator 08-23-2008, 02:24 AM
Replies: 42
Views: 31,446
Posted By Kevin McCarthy
ECO: Being on the East Coast, I had to finally...

ECO: Being on the East Coast, I had to finally pack it in last night. I see the paper clip in your attachment, but trust me, it isn't there in my Post Reply screen. I would post a screenshot,...
Forum: Polonator 08-22-2008, 08:15 PM
Replies: 42
Views: 31,446
Posted By Kevin McCarthy
ECO: "You may not post attachments", at least...

ECO: "You may not post attachments", at least according to the site. Then again, "You may not edit your posts", and I've been able to do that. I am using "Post Reply". Maybe I just can't find...
Forum: Polonator 08-22-2008, 06:15 PM
Replies: 42
Views: 31,446
Posted By Kevin McCarthy
ECO: Ahh, I just saw that I am not yet cleared...

ECO: Ahh, I just saw that I am not yet cleared to post attachments. Resolve this, and I will post the base by base protocols PDF forthwith.

I also forgot to respond to your question regarding...
Forum: Polonator 08-22-2008, 06:12 PM
Replies: 42
Views: 31,446
Posted By Kevin McCarthy
ECO: Yes, the initial chemistry is 13 bp x 2. ...

ECO: Yes, the initial chemistry is 13 bp x 2. At a more granular level, we read 7 + 6 bases from each of two tags; the reads are not contiguous, since 4-5 bases of the 17 - 18 bp tags remain...
Forum: Polonator 08-22-2008, 05:24 PM
Replies: 42
Views: 31,446
Posted By Kevin McCarthy
Samuel: Thank you for your interest in the...

Samuel: Thank you for your interest in the Polonator. We have been busy over the last few months implementing some changes to the fluidics sub-system, the flow cells, and the software. It's been a...
Forum: Polonator 07-02-2008, 10:24 AM
Replies: 42
Views: 31,446
Posted By Kevin McCarthy
I replied earlier, but for some reason it didn't...

I replied earlier, but for some reason it didn't seem to post. Tweaking of the system has taken longer than we expected, although the quality of feedback from the team at the Church Lab has been...
Forum: Polonator 04-06-2008, 04:13 AM
Replies: 42
Views: 31,446
Posted By Kevin McCarthy
Horigen: No, the elusive $1000 genome won't be...

Horigen: No, the elusive $1000 genome won't be delivered by a G.007. Perhaps the I.008 or J.008; but that would be entirely speculative at this point. We do have a road map for backwards...
Forum: Polonator 04-05-2008, 06:02 PM
Replies: 42
Views: 31,446
Posted By Kevin McCarthy
Ola: Things always go a little slower than one...

Ola: Things always go a little slower than one would wish... The initial G.007 Polonator delivered to the Church Lab in February underwent some serious tire-kicking. The Lab has very high...
Forum: Polonator 03-08-2008, 08:18 AM
Replies: 42
Views: 31,446
Posted By Kevin McCarthy
Ola: We have approximate costs determined for...

Ola: We have approximate costs determined for the reagent kits, but we can't finalize these until our volumes are nailed down, and some licensing discussions are completed. The G version is...
Forum: Polonator 02-08-2008, 07:27 AM
Replies: 42
Views: 31,446
Posted By Kevin McCarthy
pmcget, et al: Wow... those are hard ones. I'll...

pmcget, et al: Wow... those are hard ones. I'll try, but unfortunately, a lot of this is "soon come":

1. What are the consumables cost per run?
We need another week to nail this down. There is...
Forum: Polonator 02-08-2008, 04:30 AM
Replies: 42
Views: 31,446
Posted By Kevin McCarthy
It's Polonator.org, not Polonator.com

Hi everyone! I am currently attending AGBT, and am with Danaher. I worked very closely with Rich Terry and the rest of the utterly brilliant Church Lab team to create the Polonator. We shipped our...
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