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Forum: Bioinformatics 07-07-2016, 02:56 PM
Replies: 11
Views: 2,353
Posted By quattrinia
Oh-And would you recommend quality trimming...

Oh-And would you recommend quality trimming before adapter removal?
Forum: Bioinformatics 07-07-2016, 02:38 PM
Replies: 11
Views: 2,353
Posted By quattrinia
Thanks for the information. The average quality...

Thanks for the information. The average quality score of Q>30 for each sample was 59-70%. I have seen much worse than these data, so I thought this was OK! Thanks for the insight, I will definitely...
Forum: Bioinformatics 07-07-2016, 01:52 PM
Replies: 11
Views: 2,353
Posted By quattrinia
Hi, Thanks for the quick reply. This is a...

Hi,

Thanks for the quick reply. This is a denovo whole genome sequencing project of an invertebrate run with TruSeq adapters and a MiSeq Reagant kit 3. This species was multiplexed with 8 others....
Forum: Bioinformatics 07-07-2016, 10:37 AM
Replies: 11
Views: 2,353
Posted By quattrinia
I have a question regarding adapter trimming and...

I have a question regarding adapter trimming and whether you have further recommendations for adapter removal?

I have truseq indexed adapters and MiSeq reads (2*300)

I used ktrim=r k=21 mink=11...
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