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Forum: Bioinformatics 10-18-2020, 11:06 PM
Replies: 0
Views: 755
Posted By Kaskere
MEGA software

Hello,
is it possible to colorize entire phylogenetic tree clusters with MEGAX? I only found option for changing color of the branch
Forum: Bioinformatics 09-01-2020, 08:33 AM
Replies: 1
Views: 853
Posted By Kaskere
Samtools is not working

Hello,
I wrote a script for obtaining bam files, however it looks like it is not understanding flags given. I wrote a flag for no header - bS but still keep getting errors. I already feel desperated...
Forum: Bioinformatics 09-01-2020, 04:52 AM
Replies: 4
Views: 938
Posted By Kaskere
myfile.fastq.gz: gzip compressed data, from FAT...

myfile.fastq.gz: gzip compressed data, from FAT filesystem (MS-DOS, OS/2, NT)

they are compressed..

These paired end files were obtained after PE Trimmomatics
Forum: Bioinformatics 09-01-2020, 02:27 AM
Replies: 4
Views: 938
Posted By Kaskere
guzip paired fast.gz files

Hello,
I have a bunch of paired fastq.gz files, and I need to decompress them for further low quality data trimming.
However gunzip command is not working on paired files. Any suggestions?

this...
Forum: Bioinformatics 08-24-2020, 02:20 PM
Replies: 1
Views: 944
Posted By Kaskere
Segmentation fault in samtools

I am trying to generate BAM files, however there is some error with reference indexing.

[samfaipath] build FASTA index...
[E::fai_build_core] Format error, unexpected "N" at line 1
[samfaipath]...
Forum: Bioinformatics 08-24-2020, 11:32 AM
Replies: 1
Views: 928
Posted By Kaskere
bwa alignement

Hello,
I am trying to align viral RNA sequences against reference fasta file.
It looks like it is not working properly

my index code looks like that

./bwa index reference.fasta

but I keep...
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