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Forum: Sample Prep / Library Generation 06-03-2020, 08:44 AM
Replies: 1
Views: 1,117
Posted By SofiaGranja
Lightbulb TET,EBT, Tween-20

Hi everyone,

I have two questions:

There are a considerable number of DNA extraction protocols that indicate to elute in TET (10 mM Tris – HCl + 1 mM EDTA + 0.05% Tween 20). However, the...
Forum: Sample Prep / Library Generation 05-18-2020, 02:16 PM
Replies: 4
Views: 1,242
Posted By SofiaGranja
Hi! The protocol is a Blunt-End (Illumina...

Hi!

The protocol is a Blunt-End (Illumina library building) adapted from:

Kircher M, Sawyer S, Meyer M (2012) Double indexing overcomes
inaccuracies in multiplex sequencing on the Illumina...
Forum: Sample Prep / Library Generation 05-18-2020, 02:10 PM
Replies: 4
Views: 1,242
Posted By SofiaGranja
Hi! I'm using TapeStation for measurements...

Hi!

I'm using TapeStation for measurements in the region 150-1000 bp. So the Molarity (pmol/l) is automatically calculated by the TapeStation program. :)
Forum: Sample Prep / Library Generation 05-14-2020, 01:07 PM
Replies: 4
Views: 1,242
Posted By SofiaGranja
Arrow Libraries - Unusual Profiles on Tapestation

Hello,

I'm posting this hoping that you can help me understanding 3 unusual Tapestation Profiles. These profiles correspond to (DNA) libraries already indexed and purified with magnetic beads for...
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