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Forum: Sample Prep / Library Generation 07-03-2013, 06:25 AM
Replies: 1
Views: 1,243
Posted By jinpingzhang
I think it no problem. check your total RNA QC...

I think it no problem.
check your total RNA QC the 3rd image, FU is ~400, left 2 images FU just ~30, that means, them were enlarged for your vision!
Forum: Sample Prep / Library Generation 10-02-2012, 01:18 PM
Replies: 17
Views: 7,015
Posted By jinpingzhang
Cool Size selection beyond XP bead ability!

Hi, Philip,
It is very difficult or impossible using XP bead for size selection of NA. especially for small and precise NA at specific bp/nt. Also got same inoformation from Beckman Coulter.
Forum: Sample Prep / Library Generation 10-01-2012, 05:45 PM
Replies: 17
Views: 7,015
Posted By jinpingzhang
Cool Ok, we have three method for purification or...

Ok,
we have three method for purification or concentrate the NA,
1) precipitation
2) bead
3) column

precipitation will get the highest yield ratio, but time consumable, at lest 1hr.
1) 50ul...
Forum: Sample Prep / Library Generation 09-28-2012, 06:23 PM
Replies: 17
Views: 7,015
Posted By jinpingzhang
Hi, Phillip, I design this method base on...

Hi, Phillip,
I design this method base on properties of some chaotropics, like Ethanol, isopropanol,.. selection of those chaotropics let us change the range of purity flexible. for save beads, for...
Forum: Sample Prep / Library Generation 09-26-2012, 09:56 PM
Replies: 17
Views: 7,015
Posted By jinpingzhang
Cool Hi, Everyone, I think for cleanup RNA small...

Hi, Everyone,
I think for cleanup RNA small fragment just try XP bead-RNase free,

1. if you have 100ul RNA solution, add:
(1) 3M NaOAc 25ul to final contration 0.3M
(2) Isopropanol...
Forum: Sample Prep / Library Generation 09-06-2012, 08:50 PM
Replies: 3
Views: 2,588
Posted By jinpingzhang
Hi, Anjani, i do not know what kind of your...

Hi, Anjani,
i do not know what kind of your library, i try to contribute some minds.Ok, Agencourt AMPure beads is excellent product for purefication for DNA and RNA, they have RNA Clean beads for...
Forum: Sample Prep / Library Generation 09-06-2012, 08:23 PM
Replies: 1
Views: 1,398
Posted By jinpingzhang
Hi, I am not sure Illumina RNA seq library...

Hi,
I am not sure Illumina RNA seq library length and concentration. but i am sure the SOLiD RNA library some information. After your PCR and purification process, you will get several ng library,...
Forum: SOLiD 08-15-2012, 07:41 PM
Replies: 2
Views: 1,876
Posted By jinpingzhang
As my calculate, i think that is true,...

As my calculate, i think that is true, 10millinon/ul, EZ80 usually use 1430ul x 10millions. method should be same like IKA utilized, which apply 160ul same concentration beads. AB maybe give you more...
Forum: 454 Pyrosequencing 08-14-2012, 06:00 PM
Replies: 3
Views: 1,527
Posted By jinpingzhang
Yes, i think Cavaris should be better

Yes, i think Cavaris should be better
Forum: SOLiD 08-14-2012, 05:36 PM
Replies: 0
Views: 1,292
Posted By jinpingzhang
Identification of microRNA peak from Agilent samll RNA ship

Hi, everyone,
I have a question about Agilent Small RNA chip, base on Agilent show us the demo Small RNA chip's image below, I dont understand the Peak1, peak2, and peak3 means, peak2 maybe is...
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