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Forum: Illumina/Solexa 07-08-2014, 01:01 PM
Replies: 0
Views: 1,176
Posted By fadss
Post PCR-free; absolute abundance; yeast

I am new to NGS.
Goal is to find absolute abundance of yeast species in a mixed sample.
I plan to use PCR-free methods since PCR could cause amplification biased.

Plan is to use DNA fragmentase...
Forum: Illumina/Solexa 04-08-2014, 03:16 PM
Replies: 1
Views: 1,405
Posted By fadss
strains identification by STR micrsatellite using NGS

We have many vials that contain mixture of yeast STRAINS (of 3 or more strains). Currently we are using Capillaries Electrophoresis (CE). With culturing based method for isolation.
And to identify...
Forum: Illumina/Solexa 01-10-2014, 02:50 PM
Replies: 3
Views: 3,177
Posted By fadss
basically I have a vial of mix strains of...

basically I have a vial of mix strains of bacteria but a single specie. I wanted to know %proportion of each strain in a time period.

differentiate between strains by pcr-multiplex amplify 5...
Forum: Illumina/Solexa 01-10-2014, 02:24 PM
Replies: 3
Views: 3,177
Posted By fadss
Smile Fragment analysis; microsatellite or SSR/STR

Has anyone been using next gen or preferably illumina for fragment analysis.
Right now my project are running multiplex for Sanger 3130xl machine to perform fragment size analysis.
But this limited...
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