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Forum: Sample Prep / Library Generation 09-22-2014, 07:39 AM
Replies: 1
Views: 1,621
Posted By Leo Lee
I would like to attached more bioanalyzer...

I would like to attached more bioanalyzer profiles here. I have selected 3 samples from my library batch as an example.

All 3 samples are proceed in one batch. However, sample A does not have the...
Forum: Sample Prep / Library Generation 09-19-2014, 10:36 AM
Replies: 4
Views: 5,190
Posted By Leo Lee
cmbetts, thanks for your reply. Fig_A is...

cmbetts, thanks for your reply.

Fig_A is actually taken from the supernatant of original dual beads size selection (but now I change it to 1X bead purification to remove <100bp primer dimer). So...
Forum: Sample Prep / Library Generation 09-19-2014, 08:36 AM
Replies: 1
Views: 1,621
Posted By Leo Lee
NEBNext® Ultra™ DNA Library Prep QC- extra peak at around 1500bp

I used the NEBNext® Ultra™ DNA Library Prep for illumina to prepare the library and follow the standard protocol with 250ng DNA input, 8 PCR cycle and size selection for target total library size...
Forum: Sample Prep / Library Generation 09-19-2014, 08:15 AM
Replies: 4
Views: 5,190
Posted By Leo Lee
Thank you MU's suggestion. Yes, you are right...

Thank you MU's suggestion. Yes, you are right dual size selection is unnecesary, so finally I have done 1X beads purification before and after the PCR amplification using standard protocol NEBNext®...
Forum: Sample Prep / Library Generation 09-15-2014, 08:05 AM
Replies: 4
Views: 5,190
Posted By Leo Lee
Ampure XP beads Dual Bead-based Size Selection ratio and PCR purification

I want to do amplicon sequencing with HiSeq. My initiate input is PCR product with around 85bp. I decided to do library preparation by using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (Cat#...
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