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Forum: Illumina/Solexa 11-22-2017, 10:00 PM
Replies: 9
Views: 2,421
Posted By Genetic Librarian
That should not be the case, because you pool...

That should not be the case, because you pool equimolar.
Forum: Illumina/Solexa 11-22-2017, 12:10 AM
Replies: 9
Views: 2,421
Posted By Genetic Librarian
Insert size is a problem because bias works in...

Insert size is a problem because bias works in favour of smaller fragments.
So if you mix a 300 bp library and a 500 bp library 50/50, you will end up with a ~70/30 read distribution.
One reason is...
Forum: Illumina/Solexa 11-21-2017, 06:20 AM
Replies: 9
Views: 2,421
Posted By Genetic Librarian
well, mixing two libraries is a tricky task in...

well, mixing two libraries is a tricky task in general, no matter if PCR-free or not.
It works well with libraries of the same fragment size. If the two libraries are of different sizes, there is a...
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