SEQanswers

Go Back   SEQanswers > Search Forums


Showing results 1 to 5 of 5
Search took 0.00 seconds.
Search: Posts Made By: NGSMicro
Forum: Bioinformatics 09-12-2018, 06:15 AM
Replies: 2
Views: 502
Posted By NGSMicro
I don't know for certain, but it could be that...

I don't know for certain, but it could be that trimmomatic is opening 4 of the files at a time, as it has 4 threads working.
Forum: Bioinformatics 05-08-2018, 12:32 AM
Replies: 2
Views: 280
Posted By NGSMicro
Have you tried QIIME2? It provides a few...

Have you tried QIIME2?

It provides a few higher resolution methods of clustering meaning you may be able to 'get away with' using only the forward reads.
Forum: Sample Prep / Library Generation 04-26-2018, 05:56 AM
Replies: 5
Views: 2,134
Posted By NGSMicro
Also using homemade beads with the same protocol...

Also using homemade beads with the same protocol as melop, much cheaper.

If you're worried they aren't as good you can validate them against whatever you're using now.
Forum: Illumina/Solexa 04-26-2018, 05:40 AM
Replies: 6
Views: 958
Posted By NGSMicro
I have been looking at ordering my own custom...

I have been looking at ordering my own custom indices - I have hypothetically made some like this:

CAAGCAGAAGACGGCATACGAGATagctcaacGTCTCGTGGGCTCGG

Where the first section binds to the flow...
Forum: Introductions 04-26-2018, 05:32 AM
Replies: 0
Views: 713
Posted By NGSMicro
Hello all!

Read a lot of posts here whilst trying to set up some NGS methods so thought I might as well join.

We currently have a few qPCR instruments and a MiSeq.

Currently I am looking at implementing...
Showing results 1 to 5 of 5

 


All times are GMT -8. The time now is 03:16 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO