Forum: General
02-07-2012, 05:53 AM
|
|
Replies: 6
Views: 8,218
So... That's what I' m thinking to do: purify...
So... That's what I' m thinking to do: purify more total RNA, considering the degradation problem, and perform another purification step ( invisorb) before mRNA isolation (I'm guessing that some...
|
|
Forum: General
02-03-2012, 12:08 PM
|
|
Replies: 6
Views: 8,218
Hi Phillip, Thanks for the considerations.
You...
Hi Phillip, Thanks for the considerations.
You are right! The profile is mRNA mode. I also checked the RIN number of the samples and it is bellow 8 (a number that I´ve read here would be indicated)....
|
|
Forum: General
02-01-2012, 11:19 AM
|
|
Replies: 6
Views: 8,218
Hi Mellissa
I have a worse situation than...
Hi Mellissa
I have a worse situation than Nigel. My sequencing results showed 86% of rRNA ( I have to check the parameters used). The service provider showed me the mRNA agilent profile and it...
|
|
Forum: Sample Prep / Library Generation
01-10-2012, 04:11 PM
|
|
Replies: 22
Views: 11,906
|
|
Forum: RNA Sequencing
10-27-2011, 12:29 PM
|
|
Replies: 3
Views: 2,677
|
|
Forum: RNA Sequencing
10-26-2011, 11:07 AM
|
|
Replies: 3
Views: 2,677
RNA-seq depth/plant
Hi everybody!
I'm a beginner in this subject and I have a doubt about the numbers of reads / coverage necessary for plant mRNA sequencing. I would like to sequence mRNA from apical meristems of a...
|
