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Forum: Illumina/Solexa 05-02-2011, 02:36 AM
Replies: 3
Views: 2,482
Posted By busypops
Large insert libraries

Has anyone managed to make mate pair libraries with an insert size of over 5kb as supported by Illumina? Is there anything different in the circularization stage?
Forum: SOLiD 05-02-2011, 02:33 AM
Replies: 0
Views: 1,612
Posted By busypops
QC steps in Mate Pair Library Prep

Has anyone tried doing a QC step after T7 and S1 nuclease digestion in the SOLiD protocol? If so what do you do?
Thanks :)
Forum: Illumina/Solexa 01-17-2011, 04:52 AM
Replies: 1
Views: 2,485
Posted By busypops
SOLiD Mate Pair Library Prep

Has anyone tried altering the Nick Translation reaction time in the SOLiD mate pair library protocol? I need a final library sixe of around 400bp so think I need to alter the nick translation time. I...
Forum: Illumina/Solexa 01-10-2011, 05:21 AM
Replies: 3
Views: 2,546
Posted By busypops
Thanks for your reply :) I was planning on using...

Thanks for your reply :) I was planning on using the IN PE Adapter Oligo mix then using 2 indexes at PCR stage as you said. I will post and let you know how it goes.
I'm glad to hear that you were...
Forum: Illumina/Solexa 01-10-2011, 04:06 AM
Replies: 3
Views: 2,546
Posted By busypops
Indexed Mate Pair Library Prep for GAIIx/High Seq

Does anyone have any experience of preparing indexed mate pair libraries for sequencing in the GAIIx or HiSeq 2000?
I have successfully produced 3kb insert size mate pair libraries but this time I...
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