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Forum: Illumina/Solexa 05-09-2017, 07:04 AM
Replies: 15
Views: 5,836
Posted By frossit
Focus error in Miseq

We started a MiSeq running and after the first cycle there was an error: "Best focus is too near edge of range: AutoFocus would have moved z to 0, outside soft limits os -0,295001144426642......
Forum: General 10-14-2016, 07:54 AM
Replies: 1
Views: 1,377
Posted By frossit
Sequencing Ninblegen and Nextera libraries in the same lane in a Miseq

Hi !
We want to sequence 12 plasmids. 10 libraries were constructed using Ninblegen Seq Cap EZ Library SR (single index) and 2 using Nextera (dual index). We are planning to pool all these libraries...
Forum: General 10-14-2016, 07:41 AM
Replies: 2
Views: 2,152
Posted By frossit
I Can. I did it and it works.

I Can. I did it and it works.
Forum: General 10-23-2015, 05:16 AM
Replies: 2
Views: 2,152
Posted By frossit
Ninblegen SeqCap Pre and Post PCR Oligos ?

Dear Users
Do you now if i can use Post LM PCR OLigos 1&2 instead Pre LM PCR Oligos 1&2 in the Pre-Capture PCR amplification ?
Thanks
Best Regards
Fabio
Forum: Illumina/Solexa 11-26-2013, 08:39 AM
Replies: 4
Views: 2,626
Posted By frossit
Is necessary to change the configuration of...

Is necessary to change the configuration of CASAVA to demultiplex Nextera data? Is the first time we are analysing Nextera data...before that we analyzed TRuseq data.
We are doing off-line using...
Forum: Illumina/Solexa 11-26-2013, 05:22 AM
Replies: 4
Views: 2,626
Posted By frossit
How to demultiplex NEXTERA EXOME RAPID DATA run in a Hiseq 2500

Please
Somebody could help me ?
We are used to demultiplex Truseq data....but Nextera is not working ?
Thanks
Forum: General 11-05-2013, 09:40 AM
Replies: 4
Views: 3,462
Posted By frossit
The microdissected chromosome has 160 megabases....

The microdissected chromosome has 160 megabases. We are planning 2x75bp with 50X coverture sequencing protocol in a Illumina Hiseq 2500 . Can I multiplex this chr with a whole human genome in a...
Forum: General 11-04-2013, 11:07 AM
Replies: 4
Views: 3,462
Posted By frossit
Thanks for your answer !!! Im trying to help a...

Thanks for your answer !!! Im trying to help a friend of mine. I will collect more informations and post them here....
Best wishes
Fbio
Forum: General 11-04-2013, 09:56 AM
Replies: 4
Views: 3,462
Posted By frossit
Multiplexing human microdissected chromosomes with human whole genome

Hi !!!
I would like to know if i can multiplex a single human microdissected chromosome with whole human genome in a single lane of a Illumina 2500 flowcell ? Coverage of the single human chromosome...
Forum: Genomic Resequencing 10-13-2011, 06:07 AM
Replies: 2
Views: 1,715
Posted By frossit
Thanks Peter... Unfortunately the region is...

Thanks Peter...
Unfortunately the region is long.... But your suggestion is very interesting...
Im going to check if there is unique sequences between the repetitive regions... i can isolate its...
Forum: Genomic Resequencing 10-11-2011, 11:00 AM
Replies: 2
Views: 1,715
Posted By frossit
Exon Sequencing of Human Chromosome X: Im in serious trouble

Hello

My lab had discovered a locus in chromosome X that is involved with a kind of cortical malformation in a family with a pattern of X dominant inhiritance. We discovered this locus by linkage...
Forum: 454 Pyrosequencing 06-02-2011, 01:44 PM
Replies: 61
Views: 76,155
Posted By frossit
Hi Im new here Do you have any presentation...

Hi
Im new here
Do you have any presentation in ppt or pdf about Targeting sequencing or enrichment genomic regions
Thanks
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