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Forum: Ion Torrent 11-14-2017, 01:45 PM
Replies: 6
Views: 535
Posted By pfeifferr
Unfortunately, the chef uses the codes for...

Unfortunately, the chef uses the codes for tracking, and they must be unique. It will not let you accidentally re-load a previously used chip because it will recognize the barcode as used :(
Forum: Ion Torrent 11-14-2017, 09:11 AM
Replies: 6
Views: 535
Posted By pfeifferr
I inquired with lifetech, both through the sales...

I inquired with lifetech, both through the sales rep, technical support, and service engineers. All of them told me that their are no stickers available that will go on the chip that will work. The...
Forum: Ion Torrent 11-14-2017, 08:12 AM
Replies: 6
Views: 535
Posted By pfeifferr
Any way to use PGM chips without barcode on a chef/PGM?

Hello,

I had been using the Ion Torrent PGM with Ion 316 and Ion 318 chips and prepping libraries on the OT2. However, I now have an Ion CHEF, which i found out cannot use my supply of sequencing...
Forum: Bioinformatics 03-24-2014, 12:01 PM
Replies: 4
Views: 1,033
Posted By pfeifferr
Thanks Genomax, I did run the coverage...

Thanks Genomax,

I did run the coverage analysis plugin. However, this doesn't solve my real problem. I am trying to find out which bases within a set of coding exons are covered at < 20x. ...
Forum: Bioinformatics 03-24-2014, 06:55 AM
Replies: 4
Views: 1,033
Posted By pfeifferr
Trying to identify low coverage regions (bases) in my sample from a Bam and BED file.

I have a bed of my targets (start and stop coordinates of each exon of my genes of interest). I have a BAM file generated from my ION PGM run. I am trying to identify any location identified in my...
Forum: 454 Pyrosequencing 12-13-2012, 01:07 PM
Replies: 0
Views: 1,526
Posted By pfeifferr
454 GS Junior for sale (complete system)

Complete 454 GS Junior NGS Sequencing System for sale

Great benchtop next-gen sequencer for Amplicon Sequencing or Whole Genome Sequencing of microbial organisms such as bacteria, fungi, or...
Forum: 454 Pyrosequencing 12-08-2011, 12:45 PM
Replies: 27
Views: 9,759
Posted By pfeifferr
Hello, We are doing sequence capture and...

Hello,

We are doing sequence capture and then sequencing on a GS Junior. We have had many problems with the sequence capture and are trying to work that out. As far as the bead issues, we get no...
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