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Forum: RNA Sequencing 03-20-2016, 11:34 PM
Replies: 3
Views: 1,587
Posted By InDel
Generally speaking, it is preferred using WES...

Generally speaking, it is preferred using WES (whole exome seq) to identify mutations and even better if you have matched patient samples with integration of Whole Exome seq and RNA-seq to increases...
Forum: Sample Prep / Library Generation 03-17-2016, 07:17 AM
Replies: 3
Views: 1,691
Posted By InDel
There is no limit for pooling samples.

There is no limit for pooling samples.
Forum: RNA Sequencing 03-17-2016, 01:27 AM
Replies: 1
Views: 1,613
Posted By InDel
For data from public database, you always need to...

For data from public database, you always need to do data quality check to make sure you get the right input for your pipeline.
Forum: RNA Sequencing 03-16-2016, 07:33 AM
Replies: 6
Views: 1,716
Posted By InDel
1, The type of reads depends on your research...

1, The type of reads depends on your research goal. For gene expression RNA-seq, single end might be enough. For splicing, gene editing, fusion gene detection, pair end would be better.
2, More...
Forum: Sample Prep / Library Generation 03-16-2016, 06:32 AM
Replies: 3
Views: 1,440
Posted By InDel
We usually receive 50% off as a new customer for...

We usually receive 50% off as a new customer for RNA-Seq. Never received free sample.
Forum: Sample Prep / Library Generation 03-16-2016, 06:27 AM
Replies: 5
Views: 1,633
Posted By InDel
We used KAPA one. It seems to be cost effective.

We used KAPA one. It seems to be cost effective.
Forum: Sample Prep / Library Generation 03-16-2016, 06:15 AM
Replies: 7
Views: 2,951
Posted By InDel
We used Kapa kits for degraded RNA (RIN 2 or 3)...

We used Kapa kits for degraded RNA (RIN 2 or 3) and it works.
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