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Forum: Sample Prep / Library Generation 09-30-2013, 04:20 AM
Replies: 17
Views: 9,631
Posted By juelraja
Thanks a lot for your answer pmiguel! Do you...

Thanks a lot for your answer pmiguel!

Do you know if this observation is reported in literature? Is this related to zebrafish RNA or do you find similar problems with other samples also?

-Juho
Forum: Sample Prep / Library Generation 09-26-2013, 12:34 AM
Replies: 17
Views: 9,631
Posted By juelraja
Hi, I got rid of the unexpected signal in...

Hi,

I got rid of the unexpected signal in most samples by denaturating the samples in 70C for 2min (as suggested in protocol)...
Forum: Sample Prep / Library Generation 08-26-2013, 05:12 AM
Replies: 17
Views: 9,631
Posted By juelraja
Agilent Bioanalyzer: Unexpected signal in 5S region

Hi guys!

I keep having this problem with zebrafish RNA isolated with Tri Reagent. On nanodrop everything seems to be OK (260/280 ratio ~2 and 260/230 ratio >2).

Do you have any idea what can...
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