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Forum: Ion Torrent 08-22-2019, 06:27 AM
Replies: 19
Views: 2,177
Posted By Haiqu
Did you ever get a response from that Russian guy...

Did you ever get a response from that Russian guy who built his own sequencer with the Ion Torrent chip? I tried and have had no reply after three weeks.
Forum: Ion Torrent 08-22-2019, 06:13 AM
Replies: 19
Views: 2,177
Posted By Haiqu
Just following up on the SmidgION ... it will not...

Just following up on the SmidgION ... it will not be available for at least two years, according to Oxford Nanopore.
Forum: Ion Torrent 08-12-2019, 04:02 AM
Replies: 19
Views: 2,177
Posted By Haiqu
The return shipping price is built into that...

The return shipping price is built into that exorbitant $900.00 per cell. :)



Doesn't sound too bad, but you're glossing over the process needed to barcode the samples. That might well be...
Forum: Ion Torrent 08-10-2019, 07:51 PM
Replies: 19
Views: 2,177
Posted By Haiqu
If the cells can be recycled, that option should...

If the cells can be recycled, that option should be available to the purchaser. It has already been discovered that cells from the older Ion Torrent machine can be used indefinitely, and I suspect...
Forum: General 08-06-2019, 06:58 PM
Replies: 6
Views: 1,011
Posted By Haiqu
What would the ideal sequencer look like for the...

What would the ideal sequencer look like for the hobbyist?

My postulate for the required features:

1. Cheap to build, but not necessarily simple.
2. Uses readily available, relatively...
Forum: Ion Torrent 08-06-2019, 03:48 PM
Replies: 19
Views: 2,177
Posted By Haiqu
Thanks nucacidhunter. I did also receive the...

Thanks nucacidhunter. I did also receive the email. :)

I've also become aware of the unreleased ONT SmidgION device. If they can get the cost of use down it may prove to be a viable alternative,...
Forum: General 08-02-2019, 07:39 PM
Replies: 6
Views: 1,011
Posted By Haiqu
As another user pointed out, a library can be...

As another user pointed out, a library can be sent to sequencing services to be batched with other work. Until today I didn't know such services were available in Australia, they certainly don't...
Forum: Ion Torrent 08-02-2019, 07:26 PM
Replies: 19
Views: 2,177
Posted By Haiqu
:eek: Awesome!! Will PM you, thanks. They sure...

:eek: Awesome!! Will PM you, thanks. They sure don't advertise.

Edit: Oops. Forum doesn't seem to have a PM system. I've emailed you, hope it gets through.
Forum: Ion Torrent 08-02-2019, 07:21 PM
Replies: 19
Views: 2,177
Posted By Haiqu
Sheesh, another one. From the top post in this...

Sheesh, another one. From the top post in this thread:

"So the above indicates major suckage for the hobbyist."



All the ancillary stuff is available via eBay. But that's a Bad Idea (TM) for...
Forum: General 08-02-2019, 09:21 AM
Replies: 6
Views: 1,011
Posted By Haiqu
Electrophoresis: ...

Electrophoresis:

https://hackaday.com/2017/05/11/get-into-biohacking-on-the-cheap-with-this-electrophoresis-rig/
Forum: General 08-02-2019, 09:13 AM
Replies: 6
Views: 1,011
Posted By Haiqu
3D printed centrifuge: ...

3D printed centrifuge:

https://hackaday.com/2016/05/03/dna-extraction-with-a-3d-printed-centrifuge/
Forum: General 08-02-2019, 09:08 AM
Replies: 6
Views: 1,011
Posted By Haiqu
PCR machines: ...

PCR machines:

https://hackaday.io/project/1864-5-dna-replicator/details
https://openpcr.org/

Sequencer:

https://hackaday.io/project/160183-diy-dna-sequencer/details

Tutorials:
Forum: General 08-02-2019, 09:00 AM
Replies: 6
Views: 1,011
Posted By Haiqu
Homebrew gear

Doesn't seem to be a topic heading for this, maybe there should be one so that relevant data can be collected in one place.

There are several projects around for PCR's but very little else. Anyone...
Forum: Ion Torrent 08-02-2019, 08:45 AM
Replies: 19
Views: 2,177
Posted By Haiqu
My intro in the Introductions section clearly...

My intro in the Introductions section clearly states that I'm retired and consider this a hobby activity. Sorry if I seemed too knowledgeable for that to come through here, it's a side-effect of...
Forum: Ion Torrent 08-02-2019, 08:33 AM
Replies: 19
Views: 2,177
Posted By Haiqu
I live in Australia. Such services don't exist...

I live in Australia. Such services don't exist here.

Oxford Nanopore was considered and rejected on two bases: 1. Cost and short life of reagents. 2. The proprietary nature of the system. Sensors...
Forum: Personalized Genomics 08-02-2019, 08:28 AM
Replies: 1
Views: 6,073
Posted By Haiqu
I don't see the point frankly. Just get it...

I don't see the point frankly. Just get it sequenced if you need the data. On special you can get WGS sequencing for $200 these days.
Forum: Ion Torrent 08-01-2019, 03:08 PM
Replies: 19
Views: 2,177
Posted By Haiqu
You'd have to ask this guy:...

You'd have to ask this guy: https://hackaday.io/project/160183-diy-dna-sequencer/details
Forum: Ion Torrent 08-01-2019, 03:31 AM
Replies: 19
Views: 2,177
Posted By Haiqu
Why would I buy an Ion Torrent?

I've been looking at used Ion Torrent sequencers on eBay lovingly, then researching to ensure that I don't buy yet another gadget I can't use. So here's what I've discovered so far:

1. They need a...
Forum: Introductions 08-01-2019, 03:09 AM
Replies: 0
Views: 536
Posted By Haiqu
Intro

Me: Retired electronics/computer/laser/firmware engineer living on a yacht in Brisbane, Australia.

Pathway: An interest in genealogy led me to DNA testing and genetic genealogy. That then led to...
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