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Forum: Bioinformatics 05-04-2016, 10:39 AM
Replies: 0
Views: 696
Posted By mikeusyk
Fungal Primer Design

We would like to test and design fungal primers for the ITS1 regions. However the UNITE database which has a nice reference set of the ITS1/ITS2 reference sequences does not include the flanking 18S...
Forum: Bioinformatics 04-28-2016, 01:18 PM
Replies: 1
Views: 816
Posted By mikeusyk
Neandertal Genes

Hello, I am trying to download all of the genotypes of the Neandertal HLA-B gene. However I cannot seem to find this on NCBI. Is there a good repository for all available Neandertal genes?
Forum: Bioinformatics 04-04-2016, 10:04 AM
Replies: 4
Views: 2,044
Posted By mikeusyk
Thank you for your response Ryan. There I quality...

Thank you for your response Ryan. There I quality trim the reads beforehand with print-seq. I did think that it could be the misalignment in the tails that is causing the issue and actually tries...
Forum: Bioinformatics 04-04-2016, 05:48 AM
Replies: 4
Views: 2,044
Posted By mikeusyk
Bowtie (Bismark) 100% of reads fail to align

I am using bismark in order to observe the progression of methylation on specific CpG sites of different viral variants. One of the steps of bismark is to use Bowtie in order to align paired end...
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