Forum: Bioinformatics
02-06-2018, 12:05 PM
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Replies: 3
Views: 3,081
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Forum: Bioinformatics
07-12-2017, 04:27 PM
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Replies: 12
Views: 1,481
Thank you
I really appreciate your help, I will play around with it and will let you know
Many thanks
Fadi
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Forum: Bioinformatics
07-12-2017, 04:15 PM
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Replies: 12
Views: 1,481
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Forum: Bioinformatics
07-12-2017, 04:06 PM
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Replies: 12
Views: 1,481
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Forum: Bioinformatics
07-12-2017, 03:27 PM
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Replies: 12
Views: 1,481
Thank you very much SNPsaurus.
I removed...
Thank you very much SNPsaurus.
I removed the column titles for now. So I removed the ID nad the Kind from the table. WhenI use print "f0 $fields[0]\tf1 $fields[1]\n"; It prints the following
...
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Forum: Bioinformatics
07-12-2017, 03:09 PM
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Replies: 12
Views: 1,481
Hi SNPsaurus
Yes, I tried. The only way it...
Hi SNPsaurus
Yes, I tried. The only way it printed something when I put
print "fields[0] \n"
Just after the split command. I tried to play with many other things with no positive results....
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Forum: Bioinformatics
07-11-2017, 03:46 PM
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Replies: 12
Views: 1,481
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Forum: Bioinformatics
07-06-2017, 12:48 PM
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Replies: 12
Views: 1,481
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Forum: Bioinformatics
07-06-2017, 12:42 PM
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Replies: 12
Views: 1,481
Need help with Perl
Hello guys
I have the following dataset
ID Kind
259 P0_P2
260 P0_P2
57 P2
58 P2
59 P2
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Forum: Bioinformatics
01-01-2016, 08:16 AM
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Replies: 2
Views: 1,374
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Forum: Bioinformatics
12-30-2015, 08:27 AM
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Replies: 2
Views: 1,374
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Forum: Bioinformatics
11-22-2015, 04:35 PM
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Replies: 6
Views: 1,720
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Forum: Bioinformatics
11-22-2015, 09:15 AM
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Replies: 6
Views: 1,720
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Forum: Bioinformatics
11-22-2015, 05:48 AM
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Replies: 6
Views: 1,720
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Forum: Bioinformatics
11-21-2015, 12:48 PM
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Replies: 6
Views: 1,720
specific location coverage from average coverage?
Hello there,
I want to know if there is any way to calculate the coverage at a specific nucleotide across the amplicon using the average coverage, with the assumption that the reads are normally...
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Forum: Illumina/Solexa
10-30-2015, 04:13 AM
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Replies: 4
Views: 3,081
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Forum: Sample Prep / Library Generation
10-23-2015, 05:08 AM
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Replies: 3
Views: 9,131
Hello guys
If the fragmentation is...
Hello guys
If the fragmentation is following a normal distribution, shouldn't the ends be underrepresented (less coverage or the rest of the amplicon). But what is appearing in this paper is the...
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Forum: Pacific Biosciences
05-01-2015, 03:07 AM
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Replies: 2
Views: 2,786
Hi Tyson,
I thought the 3 passes will...
Hi Tyson,
I thought the 3 passes will provide enough data for building the correcting the sequencing error. However, it seems that I did not take into consideration the new sequencing chemistry....
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Forum: Pacific Biosciences
04-30-2015, 04:33 AM
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Replies: 7
Views: 2,536
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Forum: Pacific Biosciences
04-23-2015, 07:05 AM
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Replies: 7
Views: 2,536
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Forum: Pacific Biosciences
04-21-2015, 12:40 AM
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Replies: 7
Views: 2,536
Thank you very much rhall.
This is great, so...
Thank you very much rhall.
This is great, so the size selection is based on RNA classes. The protocol you mentioned is showing the size selection for maximum 6kb, is it also possible to do it for...
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Forum: Pacific Biosciences
04-18-2015, 04:59 AM
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Replies: 7
Views: 2,536
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Forum: Pacific Biosciences
01-30-2015, 03:55 PM
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Replies: 2
Views: 2,786
Targeted transcript sequencing-Iso-Seq
Hello guys,
Hi Guys,
I am preparing to use PacBio Isoforms sequencing, to sequence the total human RNA searching for specific transcripts, which are 7.4-7.7kb long, and occurring in a very...
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Forum: Pacific Biosciences
01-27-2015, 06:09 AM
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Replies: 5
Views: 2,562
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Forum: Pacific Biosciences
01-22-2015, 02:23 PM
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Replies: 5
Views: 2,562
Reference
Dear Brian, thank you for your answer.
Are you aware about any possible reference where I can find more detailed information about the process of producing forward and reverse reads, and how this...
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