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Forum: The Pipeline 11-15-2012, 06:32 PM
Replies: 80
Views: 206,764
Posted By Amiga
The grand scheme of things of for-profit sector...

The grand scheme of things of for-profit sector is targeted resequencing for diagnostic. The only thing ONT are proposing that could be useful to them is the no sample prep at all. And I am having a...
Forum: Sample Prep / Library Generation 10-26-2012, 06:52 PM
Replies: 19
Views: 10,804
Posted By Amiga
The main problem with large fragment selection is...

The main problem with large fragment selection is that the CV% can be up to 20%. So at 5kb, you have to expect +/- 1kb... Not super precise.
Forum: Sample Prep / Library Generation 04-14-2011, 08:12 PM
Replies: 19
Views: 10,804
Posted By Amiga
Hi Pauline, The worst I got was actually 30%...

Hi Pauline,

The worst I got was actually 30% and this was using a cassette that fail the test the user is supposed to do every time.

Typically I get around 70% (same target size than you or...
Forum: Sample Prep / Library Generation 03-07-2011, 10:11 AM
Replies: 19
Views: 10,804
Posted By Amiga
On the PippinPrep you use a casette, which is...

On the PippinPrep you use a casette, which is basically a fancy agarose gel, with ports to recover the eluate. The casette is disposable (and should be disposed). That's it. It supplied we the...
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