Forum: 454 Pyrosequencing
05-25-2011, 01:26 AM
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Replies: 0
Views: 1,773
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Forum: Illumina/Solexa
12-01-2008, 08:20 PM
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Replies: 3
Views: 4,264
Homemade illumina oligos and library prep kit
Hi,
I am wondering if we can use the PCR amplification primers which were made at our side.
In theory, this can work properly.
But I am worrying a little bit if there can be some problem with...
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Forum: Sample Prep / Library Generation
08-02-2008, 02:18 AM
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Replies: 18
Views: 13,354
Hi kmay,
I'm so sorry that I didn't reply to...
Hi kmay,
I'm so sorry that I didn't reply to my thread.
I was so busy to set up another application.
I'm sure that my referernce genome sequence was correct.
Could you please explain why...
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Forum: 454 Pyrosequencing
06-17-2008, 08:20 AM
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Replies: 2
Views: 2,868
High Short Reads %
Hello, there,,
Recently, I perfomed the GS FLX Sequencing run using 70X75 PTP, 4 Regions Gasket.
Unfortunately, I got some weired results.
Actual keypass reads# of my library was about ~170000,...
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Forum: Sample Prep / Library Generation
04-15-2008, 10:10 PM
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Replies: 18
Views: 13,354
ChIP-Seq problems with library generation
I am trying to sequencing ChIPed DNA.
My DNA was sonicated to 500~1kbp during IP step.
I chose two ways to solve this size problem.
Before starting library preparation for SOLEXA, one was...
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