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Forum: RNA Sequencing 03-16-2020, 11:32 AM
Replies: 7
Views: 1,214
Posted By olafblue1955
We tend to see poly-G strings in Read 2. We find...

We tend to see poly-G strings in Read 2. We find it under the %Abundant files on Basespace.
Forum: RNA Sequencing 03-16-2020, 06:51 AM
Replies: 7
Views: 1,214
Posted By olafblue1955
Hi- OK, that's fine. We know of researchers...

Hi-

OK, that's fine. We know of researchers who do the rRNA depletion as a precursor to capture as it is know that there are rRNA sequences similar to regions in mammalian RNA:

Mauro, et al....
Forum: RNA Sequencing 03-16-2020, 06:34 AM
Replies: 7
Views: 1,214
Posted By olafblue1955
Hi There- You did not mention the RNA-seq...

Hi There-

You did not mention the RNA-seq library prep method you used nor if you used in any rRNA depletion method.

What methods related to above were used?

Olaf
Forum: Sample Prep / Library Generation 02-17-2020, 05:12 AM
Replies: 1
Views: 1,208
Posted By olafblue1955
TotalScript adapters

Hello,

The adapters are basically the same 19-base sequences required for transposition of the Nextera adapter complexes into the dscDNA (5′-AGATGTGTATAAGAGACAG-3′)

I used to be in Tech Support...
Forum: Sample Prep / Library Generation 10-03-2019, 09:35 AM
Replies: 10
Views: 1,719
Posted By olafblue1955
I don't, but then again I only use the Qubit High...

I don't, but then again I only use the Qubit High Sensitivity DNA assay for measuring the quants on my dsDNA library outputs.
Forum: Sample Prep / Library Generation 10-03-2019, 07:20 AM
Replies: 10
Views: 1,719
Posted By olafblue1955
Hi, I only use Qubit for final library...

Hi,

I only use Qubit for final library quants after PCR and AMPure cleanup; I've not done DNA quants after RNA extraction (our samples are either samples that are acquired from retail sources of...
Forum: Sample Prep / Library Generation 10-03-2019, 05:58 AM
Replies: 10
Views: 1,719
Posted By olafblue1955
What RNA-seq method are you wanting to use?...

What RNA-seq method are you wanting to use? Typically, DNAse treatment in solution works fine, followed by rRNA depletion. Our library preps are done using a stranded system (any DNA reads would be...
Forum: General 06-06-2019, 04:28 AM
Replies: 3
Views: 2,178
Posted By olafblue1955
Hi Dacotah, Qiagen's PureGene kits are...

Hi Dacotah,

Qiagen's PureGene kits are nearly identical to the former Epicentre MasterPue DNA Purification kits, which are now available through Lucigen/Biosearch Technologies. Catalog numbers...
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