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Forum: Core Facilities 11-06-2014, 01:06 PM
Replies: 22
Views: 21,410
Posted By kzarn
Are there any updates here regarding good or bad...

Are there any updates here regarding good or bad reviews of the epMotion 5075 TMX? Or other models?

Thanks!
Forum: Sample Prep / Library Generation 09-24-2014, 01:56 PM
Replies: 9
Views: 3,583
Posted By kzarn
WhiteSeal - I was originally trying to fragment...

WhiteSeal - I was originally trying to fragment 52Ál of DNA in the Covaris MicroTubes (snap-tops), and was worried about sample splashing up on the sides of the tubes during fragmentation. Our...
Forum: Sample Prep / Library Generation 07-23-2014, 05:17 PM
Replies: 9
Views: 3,583
Posted By kzarn
Genomic Screen Tapes arrived today! For a (small)...

Genomic Screen Tapes arrived today! For a (small) comparison...agarose gel samples 1 and 2 are the same as Tape Station samples F2 and H2, respectively.

While the Tape Station results still...
Forum: Sample Prep / Library Generation 07-23-2014, 10:12 AM
Replies: 9
Views: 3,583
Posted By kzarn
As a side note, we will be looking to determine...

As a side note, we will be looking to determine variable SNPs, genotyping individual variation, and using individual variation to examine population structure and landscape trends. So we are hoping...
Forum: Sample Prep / Library Generation 07-23-2014, 08:48 AM
Replies: 9
Views: 3,583
Posted By kzarn
nucacidhunter - I did not see any DNA in the...

nucacidhunter - I did not see any DNA in the wells while looking at the gel on our UV transilluminator, but it would have been a bit difficult to see as I added so little sample to each well and the...
Forum: Sample Prep / Library Generation 07-22-2014, 01:08 PM
Replies: 9
Views: 3,583
Posted By kzarn
Question Degraded gDNA & TruSeq Nano Kit

Hi All,

I am hoping to prepare libraries with Illumina's TruSeq Nano kit and although Illumina recommends only using high-quality genomic DNA, I am wondering if anyone has experience using...
Forum: Sample Prep / Library Generation 06-11-2014, 09:04 AM
Replies: 2
Views: 2,914
Posted By kzarn
Thanks lorendarith! I heard back from Covaris and...

Thanks lorendarith! I heard back from Covaris and the rep said the same thing - to use the S220 protocol and to make sure that if we use the microTUBES to also use the "Rack 96 Place microTUBE" (part...
Forum: Sample Prep / Library Generation 06-09-2014, 02:39 PM
Replies: 2
Views: 2,914
Posted By kzarn
Question TruSeq Nano and Covaris E220

Does anyone have experience preparing libraries with Illumina's TruSeq Nano kit and using a Covaris E220? Illumina provides a shearing protocol for the E210, but cannot say whether the E210 protocol...
Forum: Sample Prep / Library Generation 05-23-2014, 12:57 PM
Replies: 4
Views: 3,882
Posted By kzarn
Thanks for your response, bbeitzel! Out of...

Thanks for your response, bbeitzel!

Out of curiosity, why did you decide against purchasing P-Suite (assuming you had funds available)?

In your opinion, are there any other benefits of the...
Forum: Sample Prep / Library Generation 05-23-2014, 09:56 AM
Replies: 4
Views: 3,882
Posted By kzarn
Question Automated Library Prep Instruments

I'm looking for input on Wafergen's Apollo 324 and Perkin Elmer's NGS Express.

If you have (or have used) one, do you like it? Are there any limitations that are difficult to overcome?

Has...
Forum: Sample Prep / Library Generation 05-21-2014, 12:31 PM
Replies: 3
Views: 1,726
Posted By kzarn
Are you purifying the mtDNA before starting...

Are you purifying the mtDNA before starting library preps?
Forum: Sample Prep / Library Generation 05-20-2014, 10:11 AM
Replies: 6
Views: 2,277
Posted By kzarn
I asked this question to our rep as well - here's...

I asked this question to our rep as well - here's what he had to say:

"...we recommend the Nano because it should work best for enrichment due to the fact that you want a high yield of your...
Forum: Sample Prep / Library Generation 05-20-2014, 08:36 AM
Replies: 6
Views: 2,277
Posted By kzarn
A colleague has recommended that we use the...

A colleague has recommended that we use the TruSeq PCR-free kit - do you have any reason to believe that this kit might be better or worse than the Nano kit? (Assuming we order the appropriate...
Forum: Sample Prep / Library Generation 05-19-2014, 10:12 PM
Replies: 6
Views: 2,277
Posted By kzarn
http://www.illumina.com/products/truseq-nano-dna-s...

http://www.illumina.com/products/truseq-nano-dna-sample-prep-kit.ilmn
Forum: Sample Prep / Library Generation 05-19-2014, 02:50 PM
Replies: 6
Views: 2,277
Posted By kzarn
Library prep kits and MYbaits compatibility

Hi all,

I'm wondering if anyone has any experience using Illumina's TruSeq NANO DNA kit with MYcroarray's MYbaits. The NANO kit has been recommended to us by our rep, but Illumina's website...
Forum: Sample Prep / Library Generation 05-19-2014, 12:53 PM
Replies: 2
Views: 3,290
Posted By kzarn
I have recently spoken to a few technicians and...

I have recently spoken to a few technicians and post doc about this. Everyone I know makes fresh ethanol for AMPure bead clean ups or size selection - some make 73%, others 74%, 75%, etc. (exact...
Forum: Sample Prep / Library Generation 05-19-2014, 12:34 PM
Replies: 3
Views: 1,726
Posted By kzarn
Mitochondrial Genome Sequencing

Hi All,

I am trying to determine which library prep kit to use for whole mitochondrial genome sequencing (assuming mitochondrial genomes size of ~17,000bp).

I'm currently thinking that the...
Forum: Illumina/Solexa 05-06-2014, 09:54 AM
Replies: 2
Views: 940
Posted By kzarn
Smile Thank you GenoMax!! Genohub was very...

Thank you GenoMax!!

Genohub was very helpful, and I have made an inquiry with AllSeq.

Hope you have a great day!
Forum: Illumina/Solexa 05-06-2014, 09:19 AM
Replies: 2
Views: 940
Posted By kzarn
Mitome Analysis Cost

Hi All,

I am a technician working in a wildlife genetics lab that is starting to look into sequencing protocols and my PI would like to know how much it might cost to perform whole mitome analysis...
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